| Naslov: | Digital PCR method for detection and quantification of specific antimicrobial drug-resistance mutations in human cytomegalovirus |
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| Avtorji: | ID Bogožalec Košir, Alexandra (Avtor)
ID Cvelbar, Tašja (Avtor)
ID Kammel, Martin (Avtor)
ID Grunert, Hans-Peter (Avtor)
ID Zeichhardt, Heinz (Avtor)
ID Milavec, Mojca (Avtor) |
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| Datoteke: |  PDF - Predstavitvena datoteka, prenos (5,71 MB)
MD5: 54FD3DB85846B646F8A56CCC86F678DC
 URL - Izvorni URL, za dostop obiščite https://doi.org/10.1016/j.jviromet.2020.113864
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| Jezik: | Angleški jezik |
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| Tipologija: | 1.01 - Izvirni znanstveni članek |
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| Organizacija: |  NIB - Nacionalni inštitut za biologijo
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| Povzetek: | Antimicrobial drug resistance is one of the biggest threats to human health worldwide. Timely detection and quantification of infectious agents and their susceptibility to antimicrobial drugs are crucial for efficient management of resistance to antiviral drugs. In clinical settings, viral drug resistance is most often associated with prolonged treatment of chronic infections, and assessed by genotyping methods; e.g., sequencing and PCR. These approaches have limitations: sequencing can be expensive and does not provide quantification; and qPCR quantification is hampered by a lack of reference materials for standard curves. In recent years, digital PCR has been introduced, which provides absolute quantification without the need for reference materials for standard curves. Using digital PCR, we have developed a rapid, sensitive and accurate method for genotyping and quantification of the most prevalent mutations that cause human cytomegalovirus resistance to ganciclovir. |
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| Ključne besede: | digital PCR, antimicrobial-drug resistance, HCMV, polymerase chain reaction, viruses |
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| Status publikacije: | Objavljeno |
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| Verzija publikacije: | Objavljena publikacija |
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| Datum objave: | 01.07.2020 |
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| Leto izida: | 2020 |
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| Št. strani: | str. 1-16 |
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| Številčenje: | Vol. 281, [article] 113864 |
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| PID: | 20.500.12556/DiRROS-19515  |
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| UDK: | 577 |
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| ISSN pri članku: | 0166-0934 |
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| DOI: | 10.1016/j.jviromet.2020.113864 |
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| COBISS.SI-ID: | 15879683 |
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| Datum objave v DiRROS: | 22.07.2024 |
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| Število ogledov: | 300 |
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| Število prenosov: | 212 |
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| Metapodatki: |    |
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