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Title:Do cytotoxicity and cell death cause false positive results in the in vitro comet assay?
Authors:ID Azqueta, Amaya (Author)
ID Stopper, Helga (Author)
ID Žegura, Bojana (Author)
ID Dusinska, Maria (Author)
ID Møller, Peter (Author)
Files:URL URL - Source URL, visit https://doi.org/10.1016/j.mrgentox.2022.503520
 
.pdf PDF - Presentation file, download (1,37 MB)
MD5: 47D99F39CDB17A4AF889DCDC499EE3E5
 
Language:English
Typology:1.01 - Original Scientific Article
Organization:Logo NIB - National Institute of Biology
Abstract:The comet assay is used to measure DNA damage induced by chemical and physical agents. High concentrations of test agents may cause cytotoxicity or cell death, which may give rise to false positive results in the comet assay. Systematic studies on genotoxins and cytotoxins (i.e. non-genotoxic poisons) have attempted to establish a threshold of cytotoxicity or cell death by which DNA damage results measured by the comet assay could be regarded as a false positive result. Thresholds of cytotoxicity/cell death range from 20% to 50% in various publications. Curiously, a survey of the latest literature on comet assay results from cell culture studies suggests that one-third of publications did not assess cytotoxicity or cell death. We recommend that it should be mandatory to include results from at least one type of assay on cytotoxicity, cell death or cell proliferation in publications on comet assay results. A combination of cytotoxicity (or cell death) and proliferation (or colony forming efficiency assay) is preferable in actively proliferating cells because it covers more mechanisms of action. Applying a general threshold of cytotoxicity/cell death to all types of agents may not be applicable; however, 25% compared to the concurrent negative control seems to be a good starting value to avoid false positive comet assay results. Further research is needed to establish a threshold value to distinguish between true and potentially false positive genotoxic effects detected by the comet assay.
Keywords:comet assay, cytotoxicity, genotoxicity, DNA damage, cell death
Publication status:Published
Publication version:Version of Record
Publication date:01.09.2022
Year of publishing:2022
Number of pages:str. 1-9
Numbering:Vol. 881
PID:20.500.12556/DiRROS-19349 New window
UDC:576
ISSN on article:1383-5718
DOI:10.1016/j.mrgentox.2022.503520 New window
COBISS.SI-ID:113873411 New window
Note:Ostali avtorji: Helga Stopper, Bojana Zegura, Maria Dusinska, Peter Møller; Članek v PDF formatu obsega 9 str.; First online: 23 June 2022;
Publication date in DiRROS:17.07.2024
Views:494
Downloads:339
Metadata:XML DC-XML DC-RDF
:
AZQUETA, Amaya, STOPPER, Helga, ŽEGURA, Bojana, DUSINSKA, Maria and MØLLER, Peter, 2022, Do cytotoxicity and cell death cause false positive results in the in vitro comet assay? Mutation research : Genetic toxicology and environmental mutagenesis [online]. 2022. Vol. 881, p. 1–9. [Accessed 12 April 2025]. DOI 10.1016/j.mrgentox.2022.503520. Retrieved from: https://dirros.openscience.si/IzpisGradiva.php?lang=eng&id=19349
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Record is a part of a journal

Title:Mutation research : Genetic toxicology and environmental mutagenesis
Shortened title:Mutat. res., Genet. toxicol. environ. mutagen.
Publisher:Elsevier
ISSN:1383-5718
COBISS.SI-ID:1239829 New window

Document is financed by a project

Funder:EC - European Commission
Project number:814425
Name:Risk Governance of Nanotechnology
Acronym:RiskGONE
Funder:EC - European Commission
Project number:952404
Name:Twinning towards excellence in alternative methods for toxicity assessment
Acronym:TWINALT
Funder:EC - European Commission
Project number:857381
Name:Strategies to strengthen scientific excellence and innoVation capacIty for early diagnoSIs of gastrOintestinal caNcers
Acronym:VISION
Funder:ARIS - Slovenian Research and Innovation Agency
Project number:P1-0245-2019
Name:Ekotoksiologija, toksikološka genomika in karcinogeneza
Funder:EC - European Commission
Funding programme:COST Action
Project number:CA 15132
Name:hCOMET
Funder:Other - Other funder or multiple funders
Funding programme:Spanish Ministry of Science and Innovation and the European Regional Development Fund (ERDF)
Project number:PID2020–115348RB-I00
Acronym:BIOGENSA

Licences

License:CC BY 4.0, Creative Commons Attribution 4.0 International
Link:http://creativecommons.org/licenses/by/4.0/
Description:This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.

Secondary language

Language:Slovenian
Keywords:test komet, citotoksičnost, genotoksičnost, poškodbe DNK, celična smrt


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