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Title:Fast assay to predict multipotent mesenchymal stromal cell replicative senescence dynamics
Authors:ID Kološa, Katja (Author)
ID Leskovšek, Aleš (Author)
ID Rajar, Teja (Author)
ID Lah Turnšek, Tamara (Author)
Files:URL URL - Source URL, visit https://www.future-science.com/doi/10.2144/btn-2021-0087
 
.pdf PDF - Presentation file, download (1,19 MB)
MD5: 0B81386B969524176A30F380A871DE12
 
Language:English
Typology:1.01 - Original Scientific Article
Organization:Logo NIB - National Institute of Biology
Abstract:The major obstacle to the application of mesenchymal stromal cells (MSCs) in regenerative medicine is the expansion of the donor-derived cells in vitro to obtain high cell numbers in the shortest possible time. However, MSCs gradually undergo replicative senescence after a variable number of divisions that reduce their therapeutic efficacy, which needs to be determined before administration. The authors developed a fast and simple evaluation assay testing two senescence inducers, mitoxantrone (Mxt) and trichostatin A (TSA), to predict the onset of spontaneous replicative senescence of adipose-derived mesenchymal stromal cells (ASCs) and have confirmed the correlation between induced senescence and spontaneous replicative senescence in the assay using Mxt. This protocol facilitates the standardization of therapeutic ASCs and MSCs from other origins before application.
Keywords:adipose mesenchymal stromal (stem) cells (ASCs), cell longevity, induced senescence, mesenchymal stromal (stem) cells (MSCs), mitoxantrone, replicative senescence, trichostatin A
Publication status:Published
Publication version:Version of Record
Publication date:17.02.2022
Year of publishing:2022
Number of pages:str. 1-10
Numbering:Vol. 72, no. 3
PID:20.500.12556/DiRROS-19343 New window
UDC:577.2
ISSN on article:0736-6205
DOI:10.2144/btn-2021-0087 New window
COBISS.SI-ID:100663043 New window
Publication date in DiRROS:16.07.2024
Views:525
Downloads:235
Metadata:XML DC-XML DC-RDF
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KOLOŠA, Katja, LESKOVŠEK, Aleš, RAJAR, Teja and LAH TURNŠEK, Tamara, 2022, Fast assay to predict multipotent mesenchymal stromal cell replicative senescence dynamics. Biotechniques [online]. 2022. Vol. 72, no. 3, p. 1–10. [Accessed 23 March 2025]. DOI 10.2144/btn-2021-0087. Retrieved from: https://dirros.openscience.si/IzpisGradiva.php?lang=eng&id=19343
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Record is a part of a journal

Title:Biotechniques
Shortened title:BioTechniques
Publisher:Eaton Pub. Co.
ISSN:0736-6205
COBISS.SI-ID:27332096 New window

Document is financed by a project

Funder:ARIS - Slovenian Research and Innovation Agency
Project number:P1-0245-2019
Name:Ekotoksiologija, toksikološka genomika in karcinogeneza
Funder:ARIS - Slovenian Research and Innovation Agency
Project number:J1-4247-2011
Name:Dvojna narava matičnih celic v raku in njihova uporaba v zdravljenju

Licences

License:CC BY-NC-ND 4.0, Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
Link:http://creativecommons.org/licenses/by-nc-nd/4.0/
Description:The most restrictive Creative Commons license. This only allows people to download and share the work for no commercial gain and for no other purposes.

Secondary language

Language:Slovenian
Keywords:maščobne matične celice, dolgoživost celic, mitoksantron, replikativno staranje, trihostatin A


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