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Title:Studying cell death initiation using a digital microscope
Authors:ID Arnšek, Tina (Author)
ID Golob, Nuša (Author)
ID Marondini, Nastja (Author)
ID Pompe Novak, Maruša (Author)
ID Gruden, Kristina (Author)
ID Lukan, Tjaša (Author)
Files:URL URL - Source URL, visit https://www.jove.com/t/65824/studying-cell-death-initiation-using-a-digital-microscope
 
.pdf PDF - Presentation file, download (1,13 MB)
MD5: 13C0FBCA9F2F84C1EE478248732BCCC6
 
Language:English
Typology:1.01 - Original Scientific Article
Organization:Logo NIB - National Institute of Biology
Abstract:Hypersensitive response (HR)-conferred resistance is an effective defense response that can be determined by the N resistance genes. HR is manifested as the formation of cell death zones on inoculated leaves. Here, a protocol for studying the rate of cell death initiation by imaging inoculated leaves in the time between the cell death initiation and the cell death appearance using a digital microscope is presented. The digital microscope enables a continuous imaging process in desired intervals, which allows an accurate determination of cell death initiation rate up to minutes exactly, as opposed to hours in traditional methods. Imaging with the digital microscope is also independent of light and can therefore be used during day and night without disturbing the circadian rhythm of the plant. Different pathosystems resulting in programmed cell death development could be studied using this protocol with minor modifications. Overall, the protocol thus allows simple, accurate, and inexpensive identification of cell death initiation rate.
Keywords:digital microscope, cell death, inoculated leaves
Publication status:Published
Publication version:Version of Record
Publication date:10.11.2023
Year of publishing:2023
Number of pages:str. 1-9
Numbering:Vol. 201, [article no.] ǂ3027
PID:20.500.12556/DiRROS-19271 New window
UDC:577.2
ISSN on article:1940-087X
DOI:10.3791/65824 New window
COBISS.SI-ID:177346819 New window
Note:Nasl. z nasl. zaslona; Opis vira z dne 13. 12. 2023;
Publication date in DiRROS:12.07.2024
Views:566
Downloads:266
Metadata:XML DC-XML DC-RDF
:
ARNŠEK, Tina, GOLOB, Nuša, MARONDINI, Nastja, POMPE NOVAK, Maruša, GRUDEN, Kristina and LUKAN, Tjaša, 2023, Studying cell death initiation using a digital microscope. Journal of visualized experiments [online]. 2023. Vol. 201, no. 3027, p. 1–9. [Accessed 12 April 2025]. DOI 10.3791/65824. Retrieved from: https://dirros.openscience.si/IzpisGradiva.php?lang=eng&id=19271
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Record is a part of a journal

Title:Journal of visualized experiments
Shortened title:J. vis. exp.
Publisher:MYJoVE Corporation
ISSN:1940-087X
COBISS.SI-ID:6867284 New window

Document is financed by a project

Funder:ARIS - Slovenian Research and Innovation Agency
Project number:P4-0165-2022
Name:Biotehnologija in sistemska biologija rastlin
Funder:ARIS - Slovenian Research and Innovation Agency
Project number:Z4-3217-2021
Name:Medsebojna povezanost z redoks stanjem posredovane signalizacije pri odpornosti krompirja na viruse

Licences

License:CC BY-NC-ND 3.0, Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported
Link:http://creativecommons.org/licenses/by-nc-nd/3.0/
Description:You are free to reproduce and redistribute the material in any medium or format. You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use. You may not use the material for commercial purposes. If you remix, transform, or build upon the material, you may not distribute the modified material. You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.

Secondary language

Language:Slovenian
Keywords:digitalni mikroskop, celična smrt, inokulirani listi


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