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Title:Transcription factors gene expression in chronic rhinosinusitis with and without nasal polyps
Authors:ID Soklič, Tanja (Author)
ID Rijavec, Matija (Author)
ID Šilar, Mira (Author)
ID Koren, Ana (Author)
ID Kern, Izidor (Author)
ID Hočevar-Boltežar, Irena (Author)
ID Korošec, Peter (Author)
Files:URL URL - Source URL, visit https://www.degruyter.com/downloadpdf/j/raon.ahead-of-print/raon-2019-0029/raon-2019-0029.pdf
 
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Language:English
Typology:1.01 - Original Scientific Article
Organization:Logo OI - Institute of Oncology
Abstract:Background. Chronic rhinosinusitis (CRS) current therapeutic approaches still fail in some patients with severe persistent symptoms and recurrences after surgery. We aimed to evaluate the master transcription factors gene expression levels of T cell subtypes in chronic rhinosinusitis with nasal polyps (CRSwNP) and chronic rhinosinusitis without nasal polyps (CRSsNP) that could represent new, up-stream targets for topical DNAzyme treatment. Patients and methods. Twenty-two newly diagnosed CRS patients (14 CRSwNP and 8 CRSsNP) were prospectively biopsied and examined histopathologically. Gene expression levels of T-box transcription factor (T-bet, TBX21), GATA binding protein 3 (GATA3), Retinoic acid-related orphan receptor C (RORC) and Forkhead box P3 (FOXP3) were analyzed by real-time quantitative polymerase chain reaction (RT-qPCR). Results. Eosinophilic CRSwNP was characterized by higher level of GATA3 gene expression compared to noneosinophilic CRSwNP, whereas there was no difference in T-bet, RORC and FOXP3 between eosinophilic and noneosinophilic CRSwNP. In CRSsNP, we found simultaneous upregulation of T-bet, GATA3 and RORC gene expression levels in comparison to CRSwNP; meanwhile, there was no difference in FOXP3 gene expression between CRSwNP and CRSsNP. Conclusions. In eosinophilic CRSwNP, we confirmed the type 2 inflammation by elevated GATA3 gene expression level. In CRSsNP, we unexpectedly found simultaneous upregulation of T-bet and GATA3 that is currently unexplained; however, it might originate from activated CD8+ cells, abundant in nasal mucosa of CRSsNP patients. The elevated RORC in CRSsNP could be part of homeostatic nasal immune response that might be better preserved in CRSsNP patients compared to CRSwNP patients. Further data on transcription factors expression rates in CRS phenotypes are needed.
Keywords:chronic rhinosinusitis
Publication status:Published
Publication version:Version of Record
Publication date:01.09.2019
Publisher:Association of Radiology and Oncology
Year of publishing:2019
Number of pages:str. 323-330
Numbering:Vol. 53, iss. 3
Source:Ljubljana
PID:20.500.12556/DiRROS-19169 New window
UDC:616.21
ISSN on article:1581-3207
DOI:10.2478/raon-2019-0029 New window
COBISS.SI-ID:2048522353 New window
Copyright:by Authors
Note:Soavtorji: Matija Rijavec, Mira Silar, Ana Koren, Izidor Kern, Irena Hocevar-Boltezar, Peter Korosec; Nasl. z nasl. zaslona; Opis vira z dne 26. 7. 2019;
Publication date in DiRROS:05.07.2024
Views:471
Downloads:310
Metadata:XML DC-XML DC-RDF
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SOKLIČ, Tanja, RIJAVEC, Matija, ŠILAR, Mira, KOREN, Ana, KERN, Izidor, HOČEVAR-BOLTEŽAR, Irena and KOROŠEC, Peter, 2019, Transcription factors gene expression in chronic rhinosinusitis with and without nasal polyps. Radiology and oncology [online]. 2019. Vol. 53, no. 3, p. 323–330. [Accessed 26 March 2025]. DOI 10.2478/raon-2019-0029. Retrieved from: https://dirros.openscience.si/IzpisGradiva.php?lang=eng&id=19169
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Record is a part of a journal

Title:Radiology and oncology
Publisher:Association of Radiology and Oncology
ISSN:1581-3207
COBISS.SI-ID:784507 New window

Secondary language

Language:Slovenian
Keywords:kronični rinosinuzitis


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