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Title:Recombinant human erythropoietin alters gene expression and stimulates proliferation of MCF-7 breast cancer cells
Authors:ID Trošt, Nina (Author)
ID Stepišnik, Tina (Author)
ID Berne, Sabina (Author)
ID Pucer Janež, Anja (Author)
ID Petan, Toni (Author)
ID Komel, Radovan (Author)
ID Debeljak, Nataša (Author)
Files:URL URL - Source URL, visit http://www.degruyter.com/view/j/raon.2013.47.issue-4/raon-2013-0056/raon-2013-0056.xml?format=INT
 
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Language:English
Typology:1.08 - Published Scientific Conference Contribution
Organization:Logo OI - Institute of Oncology
Abstract:Background. Functional erythropoietin (EPO) signaling is not specific only to erythroid lineages and has been confirmed in several solid tumors, including breast. Three different isoforms of erythropoietin receptor (EPOR) have been reported, the soluble (EPOR-S) and truncated (EPOR-T) forms acting antagonistically to the functional EPOR. In this study, we investigated the effect of human recombinant erythropoietin (rHuEPO) on cell proliferation, early gene response and the expression of EPOR isoforms in the MCF-7 breast cancer cell line.Materials and methods. The MCF-7 cells were cultured with or without rHuEPO for 72 h or 10 weeks and assessed for their growth characteristics, expression of early response genes and different EPOR isoforms. The expression profile of EPOR and EPOR-T was determined in a range of breast cancer cell lines and compared with their invasive properties.Results. MCF-7 cell proliferation after rHuEPO treatment was dependent on the time of treatment and the concentration used. High rHuEPO concentrations (40 U/ml) stimulated cell proliferation independently of a preceding long-term exposure of MCF-7 cells to rHuEPO, while lower concentrations increased MCF-7 proliferation only after 10 weeks of treatment. Gene expression analysis showed activation of EGR1 and FOS, confirming the functionality of EPOR. rHuEPO treatment also slightly increased the expression of the functional EPOR isoform, which, however, persisted throughout the 10 weeks of treatment. The expression levels of EPOR-T were not influenced. There were no correlations between EPOR expression and the invasiveness of MCF-7, MDA-MB-231, Hs578T, Hs578Bst, SKBR3, T-47D and MCF-10A cell lines.Conclusions. rHuEPO modulates MCF-7 cell proliferation in time- and concentration-dependent manner. We confirmed EGR1, FOS and EPOR as transcription targets of the EPO-EPOR signaling loop, but could not correlate the expression of different EPOR isoforms with the invasiveness of breast cancer cell lines.
Keywords:breast cancer, erythropoietin, gene expression
Publication status:Published
Publication version:Version of Record
Publication date:01.12.2013
Publisher:Association of Radiology and Oncology
Year of publishing:2013
Number of pages:str. 382-389
Numbering:Vol. 47, no. 4
Source:Ljubljana
PID:20.500.12556/DiRROS-18552 New window
UDC:616-006
ISSN on article:1318-2099
DOI:10.2478/raon-2013-0056 New window
COBISS.SI-ID:30880985 New window
Copyright:by Authors
Note:Izvleček v slov. na str. VI;
Publication date in DiRROS:22.03.2024
Views:948
Downloads:339
Metadata:XML DC-XML DC-RDF
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TROŠT, Nina, STEPIŠNIK, Tina, BERNE, Sabina, PUCER JANEŽ, Anja, PETAN, Toni, KOMEL, Radovan and DEBELJAK, Nataša, 2013, Recombinant human erythropoietin alters gene expression and stimulates proliferation of MCF-7 breast cancer cells. In : Articles from 7th Conference of experimental and translational oncology, [April, 20-24, 2013, Portorož] [online]. Published Scientific Conference Contribution. Ljubljana : Association of Radiology and Oncology. 2013. p. 382–389. [Accessed 21 April 2025]. Retrieved from: https://dirros.openscience.si/IzpisGradiva.php?lang=eng&id=18552
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Record is a part of a proceedings

Title:Articles from 7th Conference of experimental and translational oncology, [April, 20-24, 2013, Portorož]
COBISS.SI-ID:30907609 New window

Record is a part of a journal

Title:Radiology and oncology
Shortened title:Radiol. oncol.
Publisher:Slovenian Medical Society - Section of Radiology, Croatian Medical Association - Croatian Society of Radiology
ISSN:1318-2099
COBISS.SI-ID:32649472 New window

Secondary language

Language:Slovenian
Keywords:rak dojke, eritropoetin, izražanje genov


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