Digital repository of Slovenian research organisations

Show document
A+ | A- | Help | SLO | ENG

Title:Cysteine cathepsins, stefins and extracellular matrix degradation during invasion of transformed human breast cell lines
Authors:ID Zajc, Irena (Author)
ID Bervar, Aleš (Author)
ID Lah Turnšek, Tamara (Author)
Files:.pdf PDF - Presentation file, download (209,41 KB)
MD5: EE3B267118FA9DBEF1610EDAC302D2C7
 
Language:English
Typology:1.01 - Original Scientific Article
Organization:Logo OI - Institute of Oncology
Abstract:Background. Human breast cellular model, comprising four cell lines originating from spontaneously immortalized human breast epithelial MCF10A cell line, its c-Ha-ras transfectant, MCF10AT, and two tumourigenic derivatives, cultured from two sequential mouse xenographs, MCF10AT-Ca1a and MCF10AT-Ca1d, were used to compare the relative protein concentration of cathepsins and stefins in single cells. Methods. The relative protein concentration of cathepsins and stefins in single cells was analysed by confocal microscopy, and compared to their protein expression in cell homogenates. Results. The most invasive, MCF10AT cell line contained several fold higher protein concentration of cathepsin B and increased levels of stefins, but similar levels of cathepsin L, compared with the parental MCF10A cells. This was associated with five fold higher endocytosis of Matrigel-DQ-collagen IV (DQC) and a simultaneous increase in signal overlap between DQC and cathepsin L as well as DQC and stefin B, but a decrease in that of DQC and cathepsin B overlap in the MCF10AT cells. Simultaneously, increased signal overlaps between both cathepsins and between cathepsins-stefins pairs, were observed in this cell line. Conclusions. These results suggest that the increased collagen endocytosis and degradation in theinvasive phenotype significantly affect also the subcellular localization of cysteine cathepsins and stefins. Based on these and the reports of other authors, we hypothesize that the intracellular degradation may also be assoeiated with cathepsin L, whereas cathepsin B in the ras transformed breastcells is involved in both, the intracellular and pericellular degradation of extracellular matrix during cell migration and invasion.
Keywords:breast neoplasms, tumor cells cultured, neoplasms invasiveness, cathepsins, extracellular matrix
Publication status:Published
Publication version:Version of Record
Publication date:01.01.2006
Publisher:Association of Radiology and Oncology
Year of publishing:2006
Number of pages:str. 259-71
Numbering:Vol. 40, no. 4
Source:Ljubljana
PID:20.500.12556/DiRROS-18186 New window
UDC:577.2
ISSN on article:1318-2099
COBISS.SI-ID:1664335 New window
Copyright:by Authors
Note:42 ref.;
Publication date in DiRROS:15.02.2024
Views:718
Downloads:160
Metadata:XML DC-XML DC-RDF
:
ZAJC, Irena, BERVAR, Aleš and LAH TURNŠEK, Tamara, 2006, Cysteine cathepsins, stefins and extracellular matrix degradation during invasion of transformed human breast cell lines. Radiology and oncology [online]. 2006. Vol. 40, no. 4, p. 259–71. [Accessed 30 March 2025]. Retrieved from: https://dirros.openscience.si/IzpisGradiva.php?lang=eng&id=18186
Copy citation
  
Share:Bookmark and Share


Hover the mouse pointer over a document title to show the abstract or click on the title to get all document metadata.

Record is a part of a journal

Title:Radiology and oncology
Shortened title:Radiol. oncol.
Publisher:Slovenian Medical Society - Section of Radiology, Croatian Medical Association - Croatian Society of Radiology
ISSN:1318-2099
COBISS.SI-ID:32649472 New window

Secondary language

Language:Slovenian
Title:[Cisteinski katepsini, stefini in razgradnja izvenceličnega matriksa med invazijo človeških transformiranih celičnih linij raka dojke]


Back