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Title: A correlation of NK cytolitic test and BLT esterase test in determining activity of NK cells, stimulated by tumor target cells Authors: ID Ihan, Alojz (Author) Files: PDF - Presentation file, download (421,83 KB) MD5: 840ACDCF47C5945741BFD14CC2D6120A   Language: English Typology: 1.01 - Original Scientific Article Organization: OI - Institute of Oncology Abstract: We examined the granule exocytosis in natural killer (NK) cells by measuring N-benzyloxycarboxy-L-lysine esterase activity. As a source of NK cells we usedbuffy-coat isolated NK cells or peripheral blood lymphocytes (PBL). The exocytosis was triggered by incubating cells with ionomycin/PMA or by NK cell susceptible tumour target cells K562. When we stimulated purified NK cells with tumour target cells, a close correlation (Corr=0.84) of cytolitic NK testresults and BLT test results was obtained. We may conclude that BLT test can provide a valuable tool to discriminate further NK cell deficiency in patients with low cytolitic NK test results. Publication status: Published Publication version: Version of Record Publication date: 01.03.1999 Publisher: Slovenian Medical Association - Slovenian Association of Radiology, Nuclear Medicine Society, Slovenian Society far Radiotherapy and Oncology, and Slovenian Cancer Society Year of publishing: 1999 Number of pages: str. 27-33 Numbering: Letn. 33, št. 1 Source: Ljubljana PID: 20.500.12556/DiRROS-17909  UDC: 616-006 ISSN on article: 1318-2099 COBISS.SI-ID: 9751769  Copyright: by Authors Note: BSDOCID43302; Publication date in DiRROS: 22.01.2024 Views: 148 Downloads: 50 Metadata:     Cite this work Plain text BibTeX EndNote XML EndNote/Refer RIS ABNT ACM Ref AMA APA Chicago 17th Author-Date Harvard IEEE ISO 690 MLA Vancouver : Copy citation

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Record is a part of a journal

Title:	Radiology and oncology
Shortened title:	Radiol. oncol.
Publisher:	Slovenian Medical Society - Section of Radiology, Croatian Medical Association - Croatian Society of Radiology
ISSN:	1318-2099
COBISS.SI-ID:	32649472

Secondary language

Language:	Slovenian
Title:	Korelacija med NK citolitičnim in BLT esteraznim testom pri določevanju aktivnosti NK celic stimuliranih s tumorskimi celicami
Abstract:	Z meritvami encimske aktivnosti N-benzyloxycarboxy-L-lizinske esteraze smo preiskovali eksoitozo celic NK. Celice NK smo osamili iz levkocitnega koncentrata (Buffy coat) oz. venske krvi. Eksocitozo smo sprožili s kombinacijo PMA/ionomcin ali s tumorskimi celicami K542. Ob stimulaciji celic NK s tumorskimi celicami (K542) smo opazovali značilno soodvisnost (Cor=0.H4) med rezultati citolitične aktivnosti celic NK in med izmerjenimi encimskimi aktivnosti N-benzv- loxycarboxy-L-lizinske esteraze (BLT test). Ugotavljamo, da BLT test omogoča nadrobnej5i študij dogodkov, ki se odvijajo med citotoksično aktivnostjo celic NK.

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