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Title:Subspecies-specific sequence detection for differentiation of Mycobacterium abscessus complex
Authors:ID Minias, Alina (Author)
ID Żukowska, Lidia (Author)
ID Lach, Jakub (Author)
ID Jagielski, Tomasz (Author)
ID Strapagiel, Dominik (Author)
ID Kim, Su-Young (Author)
ID Koh, Won-Jung (Author)
ID Adam, Heather (Author)
ID Bittner, Ruth (Author)
ID Truden, Sara, Klinika Golnik (Author)
ID Žolnir-Dovč, Marija, Klinika Golnik (Author)
ID Dziadek, Jarosław (Author)
Files:.pdf PDF - Presentation file, download (1,23 MB)
MD5: A46DF102BA18581FE05EFC69C4D9A26E
 
.zip ZIP - Supplement, download (4,12 MB)
MD5: 19FA2F9CEA4C9546680DD62253778CEB
 
URL URL - Source URL, visit https://www.nature.com/articles/s41598-020-73607-x.pdf
 
Language:English
Typology:1.01 - Original Scientific Article
Organization:Logo UKPBAG - University Clinic of Respiratory and Allergic Diseases Golnik
Abstract:Mycobacterium abscessus complex (MABC) is a taxonomic group of rapidly growing, nontuberculous mycobacteria that are found as etiologic agents of various types of infections. They are considered as emerging human pathogens. MABC consists of 3 subspecies - M. abscessus subsp. bolletti, M. abscessus subsp. massiliense and M. abscessus subsp. abscessus. Here we present a novel method for subspecies differentiation of M. abscessus named Subspecies-Specific Sequence Detection (SSSD). This method is based on the presence of signature sequences present within the genomes of each subspecies of MABC. We tested this method against a virtual database of 1505 genome sequences of MABC. Further, we detected signature sequences of MABC in 45 microbiological samples through DNA hybridization. SSSD showed high levels of sensitivity and specificity for differentiation of subspecies of MABC, comparable to those obtained by rpoB sequence typing.
Keywords:Mycobacterium abscessus complex, nontuberculous mycobacteria, diagnosis
Publication status:Published
Publication version:Version of Record
Place of publishing:Velika Britanija
Publisher:Springer Nature
Year of publishing:2020
Number of pages:str. 1-9
Numbering:Vol. 10
PID:20.500.12556/DiRROS-12693 New window
UDC:579
ISSN on article:2045-2322
DOI:10.1038/s41598-020-73607-x New window
COBISS.SI-ID:38347779 New window
Copyright:© The Author(s) 2020
Note:Nasl. z nasl. zaslona; Soavtorici iz Slovenije: Sara Truden, Manca Žolnir-Dovč; Opis vira z dne 19. 11. 2020; Article no. 16415;
Publication date in DiRROS:23.11.2020
Views:12682
Downloads:1420
Metadata:XML DC-XML DC-RDF
:
MINIAS, Alina, ŻUKOWSKA, Lidia, LACH, Jakub, JAGIELSKI, Tomasz, STRAPAGIEL, Dominik, KIM, Su-Young, KOH, Won-Jung, ADAM, Heather, BITTNER, Ruth, TRUDEN, Sara, ŽOLNIR-DOVČ, Marija and DZIADEK, Jarosław, 2020, Subspecies-specific sequence detection for differentiation of Mycobacterium abscessus complex. Scientific reports [online]. 2020. Vol. 10, p. 1–9. [Accessed 26 March 2025]. DOI 10.1038/s41598-020-73607-x. Retrieved from: https://dirros.openscience.si/IzpisGradiva.php?lang=eng&id=12693
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Record is a part of a journal

Title:Scientific reports
Shortened title:Sci. rep.
Publisher:Nature Publishing Group
ISSN:2045-2322
COBISS.SI-ID:18727432 New window

Licences

License:CC BY 4.0, Creative Commons Attribution 4.0 International
Link:http://creativecommons.org/licenses/by/4.0/
Description:This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
Licensing start date:02.10.2020

Secondary language

Language:Undetermined
Keywords:kompleks Mycobacterium abscessus, netuberkulozne mikobakterije, diagnostika


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