1. Dodatne raziskave kvalifikacijskih vrst Natura 2000 ter vzpostavitev in izvajanje monitoringa ciljnih vrst rakov v letu 2010 in 2011 : končno poročiloMarijan Govedič, Matjaž Bedjanič, Al Vrezec, Ali Šalamun, 2011, final research report Abstract: Dolgoročni cilj naloge je redno pridobivanje primerljivih podatkov o stanju populacij ciljnih vrst rakov. Primarni kratkoročni cilji naloge so:
• nadaljevanje raziskave razširjenosti, na podlagi katerih bo Republika Slovenija predlagala dodatna območja za navadnega koščaka (Austropotamobius torrentium) v omrežje Natura 2000,
• zagotovitev terenskih podatkov o prisotnosti rakov za določitev območja razširjenosti za navadnega koščaka (Austropotamobius torrentium),
• oblikovanje predloga novih pSCI za navadnega koščaka (Austropotamobius torrentium),
• nadaljevanje monitoringa razširjenosti za vrsti navadni koščak (Austropotamobius torrentium) in koščenec (Austropotamobius pallipes),
• vzpostavitev populacijskega monitoringa za obe vrsti rakov.
Specifične zahteve so:
• raziskave tistih vodotokov, za katere obstajajo starejši podatki o rakih, ki v raziskavah 2006/2007 niso bili preverjeni,
• na območjih znane razširjenosti naj bodo nova vzorčna mesta vsaj 500 m dolvodno in/ali gorvodno od znanega najdišča,
• razišče se lokacije v območjih Natura 2000, kjer so raki kvalifikacijska vrsta, a na vzorčnih mestih v raziskavah 2006/2007 niso bili najdeni oz. njihova prisotnost ni bila potrjena,
• izvedba populacijskega monitoringa v štirih Natura 2000 območjih, kjer se izvede natančen popis in ocena razširjenosti.
Opomba: za potočnega raka vrste Austropotamobius pallipes uporabljamo izvirno ime koščenec, medtem ko se v uradnih dokumentih uporablja ime primorski koščak. Kljub zadnjim genetskim študijam (Pedraza-Lara s sod. 2010), ki naše koščence uvrščajo v A. italicus, ohranjamo zaenkrat (za namene tega poročila) tudi znanstveno poimenovanje A. pallipes.
Keywords: raki, monitoring
Published in DiRROS: 16.09.2024; Views: 655; Downloads: 414
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2. Vzpostavitev in izvajanje monitoringa koščaka (Austropotamobius torrentium) in koščenca (Austropotamobius pallipes) v letih 2014 in 2015 : projekt ʺVzpostavitev in izvajanje monitoringa populacij izbranih ciljnih vrst rakov v letih 2014 in 2015ʺMarijan Govedič, Aleksandra Lešnik, Vesna Grobelnik, Ali Šalamun, Al Vrezec, Martina Jaklič, Špela Ambrožič Ergaver, Andrej Kapla, 2015, final research report Abstract: V okviru naloge “Dodatne raziskave kvalifikacijskih vrst Natura 2000 ter vzpostavitev in izvajanje monitoringa ciljnih vrst rakov v letu 2010 in 2011 (končno poročilo)”, ki jo je po naročilu Ministrstva RS za okolje in prostor izvajal Center za kartografijo favne in flore s sodelovanjem Nacionalnega inštituta za biologijo in zunanjih sodelavcev (Govedič in sod. 2011) je bil predlagan okvirni načrt monitoringa potočnih rakov v Sloveniji. Večji del raziskav je bil takrat opravljen za namene opredelitve novih Natura 2000 območij za koščaka (Austropotamobius torrentium). Večina predlaganih območij iz leta 2011 (Govedič in sod. 2011) je bila v letu 2013 tudi vključena v razširjeno omrežje Natura 2000 (Uradni list RS 33/2013) in kasneje potrjena na Biogeografskem seminarju leta 2014.
V okviru sedanje naloge “Vzpostavitev in izvajanje monitoringa populacij izbranih ciljnih vrst rakov v letih 2014 in 2015” smo izdelali končno metodologijo monitoringa potočnih rakov v Sloveniji, večinoma pa smo sledili predlogu iz leta 2011 (Govedič in sod. 2011). V poročilu so predstavljeni rezultati terenskega dela. Analize populacijskih in distribucijskih trendov še niso mogoče, interpretirali pa smo nekatere izstopajoče rezultate. Glede na prejšnja poročila monitoringa (Govedič in sod. 2007, 2011) smo dopolnili glavne dejavnike ogrožanja potočnih rakov v Sloveniji s predlogi uvedbe nujnega spremljanja v Sloveniji najbolj perečih dejavnikov kot podporo upravljanju območij Natura 2000 za potočne rake.
Keywords: raki, razširjenost, Slovenija
Published in DiRROS: 16.09.2024; Views: 66; Downloads: 492
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3. Vzpostavitev in izvajanje monitoringa populacij invazivne tujerodne vrste signalni rak (Pacifastacus leniusculus) v letu 2021 : končno poročiloMarijan Govedič, Ali Šalamun, Nika Kogovšek, Al Vrezec, Matjaž Bedjanič, Stiven Kocijančič, Andrej Kapla, 2021, final research report Abstract: V Sloveniji so bile doslej registrirane štiri vrste potočnih rakov vezane na Uredbo (EU) št. 1143/2014 Evropskega parlamenta in Sveta z dne 22. oktobra 2014 o preprečevanju in obvladovanju vnosa in širjenja invazivnih tujerodnih vrst. Tri vrste, močvirski škarjar (Procambarus clarkii), trnavec (Orconectes limosus) in marmornati škarjar (Procambarus fallax f. virginalis), so bile zaznane v zadnjih štirih letih (Govedič 2017), signalni rak (Pacifastacus leniusculus) pa je prišel iz sosednje Avstrije kmalu po letu 2000. Za te vrste na območju Evropske unije od avgusta 2016 veljajo najstrožji ukrepi: prepoved trgovanja, izmenjave, gojenja in izpuščanja v okolje. Poleg omenjenih sta od tujerodnih vrst potočnih rakov v Sloveniji prisotna še rdečeškarjevec (Cherax quadricarinatus) s sicer omejeno razširjenostjo in kot tropska vrsta z nizkim potencialom širjenja (Jaklič & Vrezec 2011) ter ozkoškarjevec (Astacus leptodactylus) (Govedič & Miličič 2018). Od vseh tujerodnih vrst potočnih rakov v Sloveniji ima po doslej zbranih podatkih zaenkrat le signalni rak dejansko invazijsko populacijo.
Keywords: signalni rak, Pacifastacus leniusculus, monitoring, tujerodne vrste, Slovenija
Published in DiRROS: 04.09.2024; Views: 113; Downloads: 73
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4. Morski datlji? Ne, hvala!2010, other monographs and other completed works Abstract: Pod imenom Morski datlji? Ne, hvala! izvajamo projekt, katerega namen je ozaveščanje javnosti o načinih varovanja morskih datljev in morskega okolja ter o tem, kako nevarno za zdravje je lahko uživanje teh školjk.
Keywords: morski datlji, školjke, zavarovane vrste
Published in DiRROS: 04.09.2024; Views: 140; Downloads: 56
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5. Load testing of the first stress ribbon bridge in SloveniaĐorđe Đukić, Doron Hekič, Mirko Kosič, Rok Vezočnik, Andrej Anžlin, Andrej Štrukelj, Marjan Pipenbaher, Tomaž Weingerl, 2024, published scientific conference contribution Abstract: Paper presents selected results of the diagnostic load testing of a newly-built footbridge in Novo Mesto, Slovenia. The bridge is significant as the first bridge in Slovenia being built with the stress ribbon technology. The diagnostic load testing took place in March 2023 and comprised of three-stage static load testing, dynamic testing with a light truck driving over artificial obstacles, and measurement of ambient vibrations. The consistency between the actual response of the bridge and the response of the finite element (FE) model, used in the design, was evaluated by comparing vertical displacements from the static load testing, natural frequencies and mode shapes. The comparison of the results indicates a satisfactory agreement between the measurements and the response of the FE model, confirming the appropriateness of the employed FE model.
Keywords: bridge, bridge response measurements, stress ribon, load test
Published in DiRROS: 14.08.2024; Views: 151; Downloads: 127
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6. Identification of plasma biomarker candidates in glioblastoma using an antibody-array-based proteomic approachKlemen Zupančič, Marjan Koršič, Urška Verbovšek, Primož Rožman, Tamara Lah Turnšek, Andrej Blejec, Kristina Gruden, Helena Motaln, Miomir Knežević, Matija Veber, Ana Herman, 2014, original scientific article Abstract: Background. Glioblastoma multiforme (GBM) is a brain tumour with a very high patient mortality rate, with a median survival of 47 weeks. This might be improved by the identification of novel diagnostic, prognostic and predictive
therapy-response biomarkers, preferentially through the monitoring of the patient blood. The aim of this study was to define the impact of GBM in terms of alterations of the plasma protein levels in these patients.
Materials and methods. We used a commercially available antibody array that includes 656 antibodies to analyse
blood plasma samples from 17 healthy volunteers in comparison with 17 blood plasma samples from patients with
GBM.
Results. We identified 11 plasma proteins that are statistically most strongly associated with the presence of GBM.
These proteins belong to three functional signalling pathways: T-cell signalling and immune responses; cell adhesion and migration; and cell-cycle control and apoptosis. Thus, we can consider this identified set of proteins as potential diagnostic biomarker candidates for GBM. In addition, a set of 16 plasma proteins were significantly associated with the overall survival of these patients with GBM. Guanine nucleotide binding protein alpha (GNAO1) was associated with both GBM presence and survival of patients with GBM.
Conclusions. Antibody array analysis represents a useful tool for the screening of plasma samples for potential cancer biomarker candidates in small-scale exploratory experiments; however, clinical validation of these candidates requires their further evaluation in a larger study on an independent cohort of patients.
Keywords: glioblastoma, proteomics, biomarker
Published in DiRROS: 02.08.2024; Views: 213; Downloads: 161
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7. First record of the meagre, Argyrosomus regius (Asso, 1801), in Slovenian coastal waters with additional records from the Croatian part of the Adriatic SeaBorut Mavrič, Branko Dragičević, 2018, original scientific article Abstract: One specimen of meagre, Argyrosomus regius (Asso, 1801), was caught by trawling in the waters off Slovenia on 24th November 2016 at the depth of cca. 20 m above sandy-muddy bottom. This is the fi rst record of this species for the Slovenian part of the Adriatic Sea. Due to the fact that there are no wild populations in the Middle and North Adriatic and that remaining wild populations in the Southern Adriatic are scarce and small, the specimen is supposed to be an escapee from one of the mariculture facilities. Additional occurrences of this species from the Croatian part of the Adriatic Sea are reported.
Keywords: meagre, farm escapee, Adriatic Sea, aquaculture
Published in DiRROS: 01.08.2024; Views: 203; Downloads: 132
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9. Analysis of glioblastoma patients' plasma revealed the presence of microRNAs with a prognostic impact on survival and those of viral originKlemen Zupančič, Helena Motaln, Miomir Knežević, Urška Verbovšek, Marjan Koršič, Tamara Lah Turnšek, Primož Rožman, Matjaž Jeras, Matjaž Hren, Kristina Gruden, Andrej Blejec, Matija Veber, Ana Herman, Andrej Porčnik, Vid Podpečan, 2015, original scientific article Abstract: Background
Glioblastoma multiforme (GBM) is among the most aggressive cancers with a poor prognosis in spite of a plethora of established diagnostic and prognostic biomarkers and treatment modalities. Therefore, the current goal is the detection of novel biomarkers, possibly detectable in the blood of GBM patients that may enable an early diagnosis and are potential therapeutic targets, leading to more efficient interventions.
Experimental Procedures
MicroRNA profiling of 734 human and human-associated viral miRNAs was performed on blood plasma samples from 16 healthy individuals and 16 patients with GBM, using the nCounter miRNA Expression Assay Kits.
Results
We identified 19 miRNAs with significantly different plasma levels in GBM patients, compared to the healthy individuals group with the difference limited by a factor of 2. Additionally, 11 viral miRNAs were found differentially expressed in plasma of GBM patients and 24 miRNA levels significantly correlated with the patients’ survival. Moreover, the overlap between the group of candidate miRNAs for diagnostic biomarkers and the group of miRNAs associated with survival, consisted of ten miRNAs, showing both diagnostic and prognostic potential. Among them, hsa miR 592 and hsa miR 514a 3p have not been previously described in GBM and represent novel candidates for selective biomarkers. The possible signalling, induced by the revealed miRNAs is discussed, including those of viral origin, and in particular those related to the impaired immune response in the progression of GBM.
Conclusion
The GBM burden is reflected in the alteration of the plasma miRNAs pattern, including viral miRNAs, representing the potential for future clinical application. Therefore proposed biomarker candidate miRNAs should be validated in a larger study of an independent cohort of patients
Keywords: microRNAs, glioblastoma multiforme, biomarkers, RNA extraction, viral disease diagnosis
Published in DiRROS: 26.07.2024; Views: 244; Downloads: 108
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10. Transmembrane protein CD9 is glioblastoma biomarker, relevant for maintenance of glioblastoma stem cellsNeža Podergajs, Helena Motaln, Uroš Rajčević, Urška Verbovšek, Marjan Koršič, Nina Obad, Heidi Espedal, Miloš Vittori, Christel Herold-Mende, Hrvoje Miletic, Rolf Bjerkvig, Tamara Lah Turnšek, 2016, original scientific article Abstract: The cancer stem cell model suggests that glioblastomas contain a subpopulation of stem-like tumor cells that reproduce themselves to sustain tumor growth. Targeting these cells thus represents a novel treatment strategy and therefore more specific markers that characterize glioblastoma stem cells need to be identified. In the present study, we performed transcriptomic analysis of glioblastoma tissues compared to normal brain tissues revealing sensible up-regulation of CD9 gene. CD9 encodes the transmembrane protein tetraspanin which is involved in tumor cell invasion, apoptosis and resistance to chemotherapy. Using the public REMBRANDT database for brain tumors, we confirmed the prognostic value of CD9, whereby a more than two fold up-regulation correlates with shorter patient survival. We validated CD9 gene and protein expression showing selective up-regulation in glioblastoma stem cells isolated from primary biopsies and in primary organotypic glioblastoma spheroids as well as in U87-MG and U373 glioblastoma cell lines. In contrast, no or low CD9 gene expression was observed in normal human astrocytes, normal brain tissue and neural stem cells. CD9 silencing in three CD133+ glioblastoma cell lines (NCH644, NCH421k and NCH660h) led to decreased cell proliferation, survival, invasion, and self-renewal ability, and altered expression of the stem-cell markers CD133, nestin and SOX2. Moreover, CD9-silenced glioblastoma stem cells showed altered activation patterns of the Akt, MapK and Stat3 signaling transducers. Orthotopic xenotransplantation of CD9-silenced glioblastoma stem cells into nude rats promoted prolonged survival. Therefore, CD9 should be further evaluated as a target for glioblastoma treatment.
Keywords: biomarker, CD9, glioblastoma stem cells, neural stem cells, tetraspanin
Published in DiRROS: 26.07.2024; Views: 216; Downloads: 149
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