1. Poročilo o preskusu št.: LVG 2024-034 : vzorec št. 2024/00084Tine Hauptman, Špela Hočevar, Zina Devetak, Barbara Piškur, 2024, expertise, arbitration decision Keywords: varstvo gozdov, morfološke analize, Euwallacea fornicatus, Neocosmospora euwallaceae, strokovne naloge IVHVVR, PCR
Published in DiRROS: 23.05.2024; Views: 75; Downloads: 40
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2. Poročilo o preskusu št.: LVG 2024-043 : vzorec št. 2024/00061Tine Hauptman, Špela Hočevar, Barbara Piškur, 2024, expertise, arbitration decision Keywords: varstvo gozdov, morfološke analize, program preiskav, Fusarium circinatum, Pinus, borov smolasti rak, PCR
Published in DiRROS: 23.05.2024; Views: 78; Downloads: 0
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3. Poročilo o preskusu št.: LVG 2024-033 : vzorec št. 2024/00083Tine Hauptman, Špela Hočevar, Barbara Piškur, 2024, expertise, arbitration decision Keywords: varstvo gozdov, morfološke analize, program preiskav, Fusarium circinatum, Pinus, borov smolasti rak, PCR
Published in DiRROS: 23.05.2024; Views: 67; Downloads: 0
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4. Poročilo o preskusu št.: LVG 2024-027 : vzorec št. 2024/00058Tine Hauptman, Špela Hočevar, Barbara Piškur, 2024, expertise, arbitration decision Keywords: varstvo gozdov, morfološke analize, program preiskav, Fusarium circinatum, Pinus, borov smolasti rak, PCR
Published in DiRROS: 23.05.2024; Views: 66; Downloads: 0
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5. Poročilo o preskusu št.: LVG 2024-026 : vzorec št. 2024/00064Tine Hauptman, Špela Hočevar, Barbara Piškur, 2024, expertise, arbitration decision Keywords: varstvo gozdov, morfološke analize, program preiskav, Fusarium circinatum, Pinus, borov smolasti rak, PCR
Published in DiRROS: 23.05.2024; Views: 68; Downloads: 0
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6. Poročilo o preskusu št.: LVG 2024-031 : vzorec št. 2024/00074 in 2024/00076Nikica Ogris, Špela Hočevar, Barbara Piškur, 2024, expertise, arbitration decision Keywords: varstvo gozdov, morfološke analize, program preiskav, Fusarium circinatum, Pinus, borov smolasti rak, PCR v realnem času, Diplodia pinea
Published in DiRROS: 19.04.2024; Views: 129; Downloads: 0
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7. Poročilo o preskusu št.: LVG 2024-030 : vzorec št. 2024/00068Tine Hauptman, Špela Hočevar, Barbara Piškur, 2024, expertise, arbitration decision Keywords: varstvo gozdov, morfološke analize, program preiskav, Fusarium circinatum, Pinus, borov smolasti rak, PCR
Published in DiRROS: 19.04.2024; Views: 147; Downloads: 0
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8. Poročilo o preskusu št.: LVG 2024-029 : vzorec št.12136414Nikica Ogris, Špela Hočevar, Barbara Piškur, 2024, expertise, arbitration decision Keywords: varstvo gozdov, morfološke analize, program preiskav, Geosmithia morbida, bolezen tisočerih rakov, Juglans, PCR v realnem času
Published in DiRROS: 19.04.2024; Views: 119; Downloads: 0
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9. Poročilo o preskusu št.: LVG 2024-028 : vzorec št.10132326Tine Hauptman, Špela Hočevar, Barbara Piškur, 2024, expertise, arbitration decision Keywords: varstvo gozdov, morfološke analize, program preiskav, Fusarium circinatum, Pinus, borov smolasti rak, PCR
Published in DiRROS: 19.04.2024; Views: 129; Downloads: 0
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10. Fast and accurate multiplex identification and quantification of seven genetically modified soybean lines using six-color digital PCRAlexandra Bogožalec Košir, Sabine Muller, Jana Žel, Mojca Milavec, Allison C. Mallory, David Dobnik, 2023, original scientific article Abstract: The proliferation of genetically modified organisms (GMOs) presents challenges to GMO testing laboratories and policymakers. Traditional methods, like quantitative real-time PCR (qPCR), face limitations in quantifying the increasing number of GMOs in a single sample. Digital PCR (dPCR), specifically multiplexing, offers a solution by enabling simultaneous quantification of multiple GMO targets. This study explores the use of the Naica six-color Crystal dPCR platform for quantifying five GM soybean lines within a single six-plex assay. Two four-color assays were also developed for added flexibility. These assays demonstrated high specificity, sensitivity (limit of detection or LOD < 25 copies per reaction) and precision (bias to an estimated copy number concentration <15%). Additionally, two approaches for the optimization of data analysis were implemented. By applying a limit-of-blank (LOB) correction, the limit of quantification (LOQ) and LOD could be more precisely determined. Pooling of reactions additionally lowered the LOD, with a two- to eight-fold increase in sensitivity. Real-life samples from routine testing were used to confirm the assays’ applicability for quantifying GM soybean lines in complex samples. This study showcases the potential of the six-color Crystal dPCR platform to revolutionize GMO testing, facilitating comprehensive analysis of GMOs in complex samples.
Keywords: digital PCR, dPCR, quantification, multiplexing, genetically modified organisms, 6-color system, virus diagnostics, virology
Published in DiRROS: 29.03.2024; Views: 176; Downloads: 74
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