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Title: Tipi ektomikorize pri sadikah bukve (Fagus sylvatica L.) v rizotronih Authors: Štraus, Ines (Author) Bajc, Marko (Author) Grebenc, Tine (Author) Mali, Boštjan (Author) Kraigher, Hojka (Author) Language: Slovenian Tipology: 1.01 - Original Scientific Article Organisation: SciVie - Slovenian Forestry Institute Abstract: Naravni procesi ali dejavnost človeka vplivajo na razmere v okolju, kar se zrcali v sestavi ektomikoriznih združb. V raziskavi smo želeli ugotavljati morebitne vplive več temperaturnih režimov zraka in tal (substrata) na pojavljanje in vrstno pestrost tipov ektomikorize na koreninskih vršičkih sadik bukve in v substratu. V ta namen smo analizirali pojavljanje tipov ektomikorize na sadikah bukve v rizotronih, izpostavljenih štirim temperaturnim razmeram v okolju: 1.) zrak 15-25 °C; 2.) zrak 15-25 °C v kombinaciji s hlajenim koreninskim sistemom za 5 °C; 3.) povišana temperatura zraka od 30-50 °C in 4.) zunanja (ambientalna) temperatura zraka v Ljubljani. Tipe ektomikorize smo identificirali s kombiniranim pristopom po anatomsko morfoloških znakih in z analizo molekularnih markerjev (ITS1-5.8S rDNK-ITS2 ribosomalna regija v rDNK). Na 51 koreninskih vršičkih 40 sadik smo identificirali 6 tipov ektomikorize. Pestrost ektomikoriznih tipov je bila največja pri razmerah blizu optimalnih za rast bukve. Po vrstni sestavi združbe ektomikoriznih gliv sta si najbolj podobna poskusa s temperaturo zraka15-25 °C z ali brez hlajenja koreninskega sistema. Najpogostejša ektomikorizna vrsta je bila Hebeloma sacchariolens, ki je bila tudi najpogosteje opažena vrsta na drobnih koreninah odmrlih sadik. Izbrane vrste ektomikoriznih gliv smo z metodo DGGE dokazali tudi v vseh analiziranih vzorcih substrata. Keywords: sadike bukve, ektomikoriza, temperatura zraka, temperatura substrata, molekularni markerji, filogenija Year of publishing: 2011 COBISS_ID: 3256998  UDC: 630*16/17:630*23(045)=163.6 ISSN on article: 0351-3114 OceCobissID: 6206978  URN: URN:NBN:SI:doc-DHAZSOAJ Views: 3124 Downloads: 1127 Files: PDF - Presentation file, download (1,11 MB)   Journal: Zb. gozd. lesar. Gozdarski inštitut Slovenije   Metadata:     Cite this work BibTeX EndNote XML EndNote/Refer RIS ABNT ACM Ref AMA APA Chicago Harvard IEEE ISO 690 MLA Vancouver :

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Language:	English
Title:	Types of ectomycorrhizae on beech seedlings (Fagus sylvatica L.) in rhizotrons
Abstract:	Natural processes or human activities affect environmental conditions, as reflected in the structure of the communities and the level of ectomycorrhizalfungi. The aim of the study was to determine the potential impacts of several temperature regimes of air and soil (substrate) on the occurrence and species diversity of ectomycorrhizal fungi in symbiosis and in the substrate. For this purpose, we analyzed the occurrence of types of ectomycorrhizae on beech seedlings in rhizotrons exposed to four different environmental temperature conditions: 1) air 15-25°C, 2) air 15-25°C in combination with cooling of roots for 5°C, 3) elevated air temperature from 30-50°C, and 4) ambiental conditions (air temperature in Ljubljana). Types of ectomycorrhizae were identified with a combined approach of anatomical morphological characterisation and with analysis of molecular markers (ITS1-5.8S rDNA-ITS2 ribosomal region in rDNA). We identified a total of 6 types of ectomycorrhizae on 51 root tips on 40 beech seedlings. The diversity of ectomycorrhizal types was the highest in terms of near-optimal growth conditions of beech. Most similar as far as species diversity of ectomycorrhizal fungi are concerned were the experiments with air temperature of 15-25°C with or without cooling of the root system. The commonest ectomycorrhizal species was Hebeloma sacchariolens, which was also the most commonly seen species in fine roots of dead plants. Selected species of ectomycorrhizal fungi were further substantiated with DGGE method in all analyzed substrate samples.

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