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Title:Okužba s pripravki CCB zaščitenih izpranih lesnih vzorcev z glivami razkrojevalkami lesa
Authors:ID Humar, Miha (Author)
ID Pohleven, Franc (Author)
Files:.pdf PDF - Presentation file, download (232,11 KB)
MD5: 72A146FD93A23F45CCB0B4BECE46F979
PID: 20.500.12556/dirros/6f9ffb6c-0e4a-42a1-9f22-5caf5c960a27
 
Language:Slovenian
Typology:1.01 - Original Scientific Article
Organization:Logo SciVie - Slovenian Forestry Institute
Abstract:Les, zaščiten s pripravki CCB, je odporen proti trohnjenju. Vendar v zadnjih letih opažamo, da se na lesu, impregniranem s CCB, pojavljajo okužbe z glivami, tolerantnimi na Cu. V raziskavi smo opazovali preraščanje in penetracijo hif na zaščitenih vzorcih, izpostavljenim glivam razkrojevalkam, in osvetlili vpliv izpiranja na kolonizacijo. Vzorce smo izdelali iz beljave smrekovine (Picea abies) in jih impregnirali s 5 % raztopino CCB v skladu s standardom SIST EN 113. Del kondicioniranih vzorcev smo izpirali po standardu SIST EN 84. V tretjino izpranih in neizpranih vzorcev smo vzdolžno zvrtali luknjo in vanjo vstavili palčko (r = 1,5 mm, l = 25 mm). Nato smo odprtino zatesnili z epoksidnim premazom. Sterilizirane impregnirane in neimpregnirane vzorce smo izpostavili dvema na baker tolerantnima sevoma (Antrodia vaillantii in Leucogyrophana pinastri) in dvema na baker občutljivima izolatoma (Poria monticola, Gloeophyllum trabeum). Po izpostavitvi smo iz vzorcev previdno odstranili palčko in jo postavili na sterilno hranilno gojišče, kjer smo dva tedna opazovali prisotnost hif. Stopnjo kolonizacije smo ovrednotili tudi z merjenjem CO2. Po 16 tednih izpostavitve smo ugotovili še izgubo mase izpostavljenih vzorcev. Neimpregnirane vzorce so najhitreje prerasle hife glive G. trabeum. Po drugi strani pa pri vzorcih, impregniranih s CCB, na palčkah tudi po 12 tednih izpostavitve nismo zasledili prisotnosti hif. Prodiranje hif pri izpranih impregniranih vzorcih je bilo bistveno hitrejše. Menimo, da je razlog za intenzivnejšo kolonizacijo izpranih vzorcev, impregniranih s CCB, izprani bor, ki v nižjih koncentracijah ni zaviral preraščanja micelija.
Keywords:toleranca na baker, CCB, les, glive razkrojevalke, bor, izpiranje, preraščanje, aspiracija
Publication status:Published
Publication version:Version of Record
Year of publishing:2003
Number of pages:str. 125-136
Numbering:Vol. 71
PID:20.500.12556/DiRROS-7581 New window
UDC:630*841.4
ISSN on article:0351-3114
URN:URN:NBN:SI:doc-EQNDWPS1
COBISS.SI-ID:1123209 New window
Publication date in DiRROS:12.07.2017
Views:4849
Downloads:2039
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Record is a part of a journal

Title:Zbornik gozdarstva in lesarstva. forest and wood science & technology
Shortened title:Zb. gozd. lesar.
Publisher:Gozdarski inštitut Slovenije, Biotehniška fakulteta, Oddelek za gozdarstvo in obnovljive gozdne vire, Biotehniška fakulteta, Oddelek za lesarstvo
ISSN:0351-3114
COBISS.SI-ID:6206978 New window

Secondary language

Language:English
Title:Infection of CCB preserved and leached wood specimens with wood decay fungi
Abstract:CCB treated wood is generally resistant to all wood decay fungi. However, like at CCA impregnated wood, susceptibility of CCB treated wood to copper tolerant fungi have been observed. It was investigated whether the hyphae of brown rot fungi could overgrow on and penetrate into the wood samples. Samples made of Norway spruce (Picea abies) were impregnated with 5 % CCB solution according to the EN 113 procedure. After conditioning, part of the samples was leached according to the EN 84 method. A small stick of unimpregnated wood (r = 1.5 mm, l = 25 mm) was inserted into a hole, bored in the center of the sample, and then sealed with epoxy coating. Sterilized, leached and non-leached impregnated and unimpregnated specimens were exposed to two copper-tolerant (Antrodia vaillantii, Leucogyrophana pinastri) and two copper sensitive (Poria monticola, Gloeophyllum trabeum) brown rot fungal strains. After exposure, the inserted wood pieces were removed from the specimens and put onto nutrient medium in petri dishes. Growth of the hyphae from those wood pieces was then visually determined. Rate of colonization by the fungi was determined by measurement of CO2. Mass losses after 16 weeks of exposure were also determined. The fastest colonization of the unimpregnated specimens was by G. trabeum. On the other hand, no fungal growth could be detected on non-leached CCB impregnated specimens even after 12 weeks of exposure. However, significantly more intense colonization by the copper tolerant fungi was detected on the leached CCB treated samples. We concluded that the reason for observed higher susceptibility originates in leached boron, which did not influence, in lower concentrations, fungal growth.
Keywords:copper tolerance, CCB, wood, wood decay fungi, leaching, boron, overgrow, respiration


Collection

This document is a part of these collections:
  1. Acta Silvae et Ligni

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