Digital repository of Slovenian research organisations

Show document
A+ | A- | SLO | ENG

Title:Design principles for rapid folding of knotted DNA nanostructures
Authors:Kočar, Vid (Author)
Schreck, John S. (Author)
Čeru, Slavko (Author)
Gradišar, Helena (Author)
Bašić, Nino (Author)
Pisanski, Tomaž (Author)
Doye, Jonathan P. K. (Author)
Jerala, Roman (Author)
Tipology:1.01 - Original Scientific Article
Organisation:Logo KI - National Institute of Chemistry
Abstract:Knots are some of the most remarkable topological features in nature. Self-assembly of knotted polymers without breaking or forming covalent bonds is challenging, as the chain needs to be threaded through previously formed loops in an exactly defined order. Here we describe principles to guide the folding of highly knotted single-chain DNA nanostructures as demonstrated on a nano-sized square pyramid. Folding of knots is encoded by the arrangement of modules of different stability based on derived topological and kinetic rules. Among DNA designs composed of the same modules and encoding the same topology, only the one with the folding pathway designed according to the "free-end" rule folds efficiently into the target structure. Besides high folding yield on slow annealing, this design also folds rapidly on temperature quenching and dilution from chemical denaturant. This strategy could be used to design folding of other knotted programmable polymers such as RNA or proteins.
Year of publishing:2016
COBISS_ID:5880858 Link is opened in a new window
ISSN on article:2041-1723
OceCobissID:2315876 Link is opened in a new window
DOI:10.1038/ncomms10803 Link is opened in a new window
Note:Sodelavci: John S. Schreck, Slavko Čeru, Helena Gradišar, Nino Bašić, Tomaž Pisanski, Jonathan P. K. Doye & Roman Jerala; Opis vira z dne 7. 3. 2016; Nasl. z nasl. zaslona; Št. članka 10803;
Files:URL URL - Presentation file, visit
Journal:Nature communications
Nature Publishing Group
Hover the mouse pointer over a document title to show the abstract or click on the title to get all document metadata.


License:CC BY 4.0, Creative Commons Attribution 4.0 International
Description:This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
Licensing start date:13.04.2016