| Title: | Cytopathological assessment is an accurate method for identifying immunophenotypic features and BRCA1/2 mutations of high-grade serous carcinoma from ascites |
|---|
| Authors: | ID Miceska, Simona (Author)
ID Škof, Erik (Author)
ID Novaković, Srdjan (Author)
ID Stegel, Vida (Author)
ID Jeričević, Anja (Author)
ID Grčar-Kuzmanov, Biljana (Author)
ID Smrkolj, Špela (Author)
ID Cvjetičanin, Branko (Author)
ID Bebar, Sonja (Author)
ID Globočnik Kukovica, Marta (Author)
ID Kloboves-Prevodnik, Veronika (Author) |
|---|
| Files: |  PDF - Presentation file, download (795,45 KB)
MD5: 05DEFAB5564E106FA0A44DA4F3AB69FF
 URL - Source URL, visit https://acsjournals.onlinelibrary.wiley.com/doi/epdf/10.1002/cncy.22664
|
|---|
| Language: | English |
|---|
| Typology: | 1.01 - Original Scientific Article |
|---|
| Organization: |  UKC LJ - Ljubljana University Medical Centre
|
|---|
| Abstract: | High‐grade serous carcinoma (HGSC) is the most common and aggressive type of ovarian cancer, and it is often associated with ascites at presentation. The objective of this study was to evaluate the accuracy of cytopathology to identify immunophenotypic features of HGSC and BRCA1/2 mutations from ascites. Methods: The study included 45 patients with histologically confirmed primary HGSC and malignant ascites. Immunocytochemistry (ICC) staining for PAX8, WT1, P53, P16, and Ki‐67 was performed on cytospins and cytoblocks prepared from ascites. Next‐generation sequencing (NGS) was used to detect germline/somatic BRCA1/2 mutations in the ascites. Both ICC and NGS results were compared with immunohistochemistry (IHC) and NGS results from tissue blocks of primary tumor. Cronbach α and χ2 statistics, respectively, were used. Results: ICC/IHC results for PAX8, WT1, P53, and P16 showed good reliability between cytospins, cytoblocks, and tissue blocks (α > 0.75), whereas poor reliability and significant differences were observed for Ki‐67 between ascites and tissue blocks (α < 0.26; p < .001 [Kruskal–Wallis]). For germline BRCA1/2 mutations, 100% concordance was confirmed, but only 14% concordance was confirmed for somatic mutations. Conclusions: These results demonstrate that cytopathology is an accurate method for identifying immunophenotypic features of HGSC and detecting germline BRCA1/ 2 mutations from ascites. However, further investigation is required for assessing the proliferation activity of HGSC in ascites and for detecting somatic BRCA1/2 mutations. |
|---|
| Keywords: | ascites, BRCA1/2 mutations, cytopathology, high-grade serous carcinoma |
|---|
| Publication status: | Published |
|---|
| Publication version: | Version of Record |
|---|
| Year of publishing: | 2023 |
|---|
| Number of pages: | str. 188-197 |
|---|
| Numbering: | Vol. 131, iss. 3 |
|---|
| PID: | 20.500.12556/DiRROS-31684  |
|---|
| UDC: | 616-07 |
|---|
| ISSN on article: | 1934-662X |
|---|
| DOI: | 10.1002/cncy.22664 |
|---|
| COBISS.SI-ID: | 131704067 |
|---|
| Publication date in DiRROS: | 06.08.2026 |
|---|
| Views: | 150 |
|---|
| Downloads: | 112 |
|---|
| Metadata: |    |
|---|
|
:
|
Copy citation |
|---|
| | | | Share: |  |
|---|
Hover the mouse pointer over a document title to show the abstract or click
on the title to get all document metadata. |
