Show document

A+ | A- | | SLO | ENG

Title: Primerjava dveh metod za merjenje listnih rež pri dobu in gradnu Authors: ID Mrak, Tanja (Author) ID Gričar, Jožica (Author) Files: URL - Source URL, visit https://ojs.sazu.si/folia_bio_geo/article/view/8092/7560   PDF - Presentation file, download (492,54 KB) MD5: 29198B74298D1C6E20DB3CF544D70F30   Language: Slovenian Typology: 1.01 - Original Scientific Article Organization: SciVie - Slovenian Forestry Institute Abstract: Lastnosti listnih rež, kot sta gostota in velikost, pomembno vplivajo na izmenjavo plinov, zato je njihova analiza ključna za razumevanje fiziologije rastlin, njihove prilagodljivosti na okoljske razmere ter odzivov na podnebne spremembe. Z našo raziskavo smo želeli ugotoviti, ali ročne meritve listnih rež pri drevesnih sadikah lahko nadomestimo z avtomatizi-ranimi meritvami, kar bi zaradi prihranka časa močno pove-čalo število vzorcev, ki jih lahko analiziramo. Pripravili smo odtise spodnje povrhnjice z listnimi režami pri sadikah doba in gradna z istega rastišča. Na desetih naključno izbranih po-snetkih preparatov pod 200 x in 400 x povečavo mikroskopa za vsako vrsto smo merili število režnih kompleksov ter dol-žino in širino celic zapiralk ter porusa z ročnimi meritvami v programu Image J ter z avtomatiziranimi meritvami v pro-gramu StoManager1. Rezultate smo statistično ovrednotili. Pri meritvah števila režnih kompleksov nismo zasledili stati-stično značilnih razlik med obema metodama. Bland-Altma-nova analiza je pokazala dobro ujemanje med ročnimi in av-tomatiziranimi meritvami. Avtomatizirane meritve so sicer rahlo precenile število režnih kompleksov, vendar je bila na-paka majhna. Pri meritvah velikosti so bile razlike med obema metodama meritev statistično značilne, Bland-Altma-nova analiza pa je pokazala odstopanja med obema metoda-ma pri večjih razponih velikosti merjenih struktur. Z regre-sijsko analizo smo ugotovili, da je ujemanje med ročnimi in avtomatiziranimi meritvami v velikostnem razredu 20-25 μm (večjih struktur nismo merili) dokaj zanesljivo, z manjša-njem velikosti struktur pa upade, najverjetneje zaradi se-gmentacije slike pri avtomatizirani metodi, pri kateri je za-znavanje robov manj natančno. Pri majhnih strukturah že majhne razlike v segmentaciji privedejo do večjih napak. Po drugi strani pa lahko tudi ročne meritve na mikroskopskih slikah postanejo vse bolj subjektivne, ko se velikost struktur zmanjšuje. Naši rezultati nakazujejo, da so avtomatizirane meritve s programom StoManager1 ustrezna nadomestitev ročnih meritev števila oz. gostote režnih kompleksov ter veli-kosti večjih struktur znotraj režnega kompleksa za izbrane drevesne vrste. Keywords: oblika listnih rež, orodja za analizo slike, avtomatizirane meritve, sadike, hrasti, Quercus robur, Quercus petraea, število listnih rež Publication version: Version of Record Publication date: 01.01.2026 Year of publishing: 2026 Number of pages: str. 21-32 Numbering: Vol. 67, iss. 1 PID: 20.500.12556/DiRROS-31294  UDC: 630*16 ISSN on article: 2335-2914 DOI: 10.3986/f bg0119  COBISS.SI-ID: 285956355  Note: Nasl. z nasl. zaslona; Opis vira z dne 24. 7. 2026; Publication date in DiRROS: 24.07.2026 Views: 41 Downloads: 19 Metadata:    Cite this work Plain text BibTeX EndNote XML EndNote/Refer RIS ABNT ACM Ref AMA APA Chicago 17th Author-Date Harvard IEEE ISO 690 MLA Vancouver : Copy citation

Share: Hover the mouse pointer over a document title to show the abstract or click on the title to get all document metadata.

Record is a part of a journal

Title:	Folia biologica et geologica
Publisher:	Slovenska akademija znanosti in umetnosti
ISSN:	2335-2914
COBISS.SI-ID:	35019053

Licences

License:	CC BY-NC-SA 4.0, Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International
Link:	http://creativecommons.org/licenses/by-nc-sa/4.0/
Description:	A Creative Commons license that bans commercial use and requires the user to release any modified works under this license.

Secondary language

Language:	English
Title:	Comparison of two methods for measurement of stomata in pedunculate and sessile oak
Abstract:	Stomatal traits, such as density and size, significantly inf lu-ence gas exchange; therefore, their analysis is essential for un-derstanding plant physiology, their adaptation to environmen-tal conditions, and their responses to climate change. The aim of this study was to determine whether manual measurements of stomata in tree seedlings could be replaced by automated measurements, which would substantially increase the num-ber of samples that can be analysed due to time savings. Epi-dermal imprints of stomata were prepared from seedlings of pedunculate oak and sessile oak growing at the same site. Ten randomly selected micrographs of the preparations at 200 × and 400 × magnification per species were used to analyse the number of stomata as well as the length and width of guard cells and stomatal pore, using both manual measurements in ImageJ and automated measurements in StoManager1. The re-sults were statistically evaluated. No statistically significant differences between the two methods were found for measure-ments of stomatal number. Bland–Altman analysis showed good agreement between manual and automated measure-ments. Although the automated method slightly overestimated the number of stomata, the error was small. for size measure-ments, statistically significant differences between the two methods were observed, and Bland–Altman analysis revealed deviations between the methods at larger size ranges of the measured structures. Regression analysis indicated that agree-ment between manual and automated measurements was rea-sonably reliable within the size range of 20–25 μm (larger structures were not measured), but decreased with decreasing structure size, most likely due to image segmentation in the automated method and the associated less precise edge detec-tion. In small structures, even minor segmentation differences can lead to larger errors. On the other hand, manual measure-ments in microscopy images can also become increasingly sub-jective as the size of structures decreases. Our results suggest that automated measurements using StoManager1 represent a suitable alternative to manual measurements of stomatal num-ber/ density and dimensions of larger structures within the stomatal complex for the selected tree species.
Keywords:	number ofleaf stomata, leaf stomata shape, image analysis tools, automated measurements, seedlings, oaks, Quercus robur, Quercus petrae

Back