| Title: | Introduction of a spectrophotometric method for salivary iodine determination on microplate based on Sandell-Kolthoff reaction |
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| Authors: | ID Oblak, Adrijana (Author)
ID Imperl, Jernej (Author)
ID Kolar, Mitja (Author)
ID Marolt, Gregor (Author)
ID Krhin, Blaž (Author)
ID Zaletel, Katja (Author)
ID Gaberšček, Simona (Author) |
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| Files: |  URL - Source URL, visit https://sciendo.com/article/10.2478/raon-2024-0035
 PDF - Presentation file, download (763,51 KB)
MD5: 86A497BC695BC4403DF77544B9F36054
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| Language: | English |
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| Typology: | 1.01 - Original Scientific Article |
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| Organization: |  DRO - Association of Radiology and Oncology
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| Abstract: | Background. Iodine is an essential element for the synthesis of thyroid hormones. Therefore, a reliable marker of iodine supply is important. Iodine is predominantly excreted via kidneys, but also via salivary glands. Our aim was to introduce a new and simple method for determination of salivary iodine concentration (SLIC). Materials and methods. Self-prepared chemicals and standards for Sandell-Kolthoff reaction on microplate with ammonium peroxydisulfate (AP) in the range 0−400 µg/L were used. Suitability of water-based standards (WBS) and artificial saliva-based standards (ASS) for standard curve were tested. We followed standards for method validation, defined concentration of used AP and compared our results with Inductively Coupled Plasma Mass Spectrometry (ICP-MS). Results. WBS gave more reliable results than ASS as an underestimation of iodine concentration was found for ASS. LoB was 6.5 µg/L, LoD 12.0 µg/L, therefore analytical range was 12−400 µg/L. Intra- and inter-assay imprecisions at iodine concentrations, namely 20, 100, 165, and 350 µg/L were 18.4, 5.1, 5.7, and 2.8%, respectively, and 20.7, 6.7, 5.1, and 4.3%, respectively. Suitable molarity of AP was 1.0 mol/L and showed no difference to 1.5 mol/L (P values for samples with concentration 40, 100, and 150 µg/L, were 0.761, 0.085, and 0.275, respectively), whereas there was a significant change using 0.5 mol/L (P<0.001). Saliva samples could be diluted up to 1:8. There was no interference of thiocyanate and caffeine up to 193.5 mg/L. Our original method was comparable to ICP-MS. Spaerman coefficient was 0.989 (95% CI: 0.984−0.993). Conclusions. The new method for SLIC determination is in excellent agreement with ICP-MS and easy-to-use. |
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| Keywords: | iodine, salivary iodine concentration, Sandell-Kolthoff reaction |
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| Publication status: | Published |
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| Publication version: | Version of Record |
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| Publication date: | 01.09.2024 |
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| Publisher: | Association of Radiology and Oncology |
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| Year of publishing: | 2024 |
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| Number of pages: | str. 357-365, V |
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| Numbering: | Vol. 58, no. 3 |
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| Source: | Ljubljana |
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| PID: | 20.500.12556/DiRROS-30466  |
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| UDC: | 61 |
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| ISSN on article: | 1318-2099 |
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| DOI: | 10.2478/raon-2024-0035 |
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| COBISS.SI-ID: | 204612355 |
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| Note: | Soavtorji: Jernej Imperl, Mitja Kolar, Gregor Marolt, Blaz Krhin, Katja Zaletel, Simona Gaberscek;
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| Publication date in DiRROS: | 26.06.2026 |
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| Views: | 33 |
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| Downloads: | 23 |
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