| Title: | A novel digital PCR assay for detection and comprehensive characterization of Molluscum contagiosum virus genotypes MOCV1, MOCV2, and MOCV3 and recombinant lineages |
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| Authors: | ID Zorec, Tomaž Mark (Author)
ID Skubic, Lucijan (Author)
ID Poljak, Mario (Author) |
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| Files: |  PDF - Presentation file, download (589,05 KB)
MD5: D6379888FB66C343F5B9CCC473591426
 URL - Source URL, visit https://www.sciencedirect.com/science/article/pii/S0166093424001174
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| Language: | English |
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| Typology: | 1.01 - Original Scientific Article |
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| Organization: |  UKC LJ - Ljubljana University Medical Centre
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| Abstract: | Molluscum contagiosum virus (MOCV) is an important human pathogen causing a high disease burden worldwide. It is the last exclusively human-infecting poxvirus still circulating in its natural reservoir-a valuable model of poxviral evolution. Unfortunately, MOCV remains neglected, and little is known about its evolutionary history and circulating genomic variants, especially in non-privileged countries. The design weaknesses of available MOCV detection/genotyping assays surfaced with recent accumulation of abundant sequence information: all existing MOCV assays fail at accurate genotyping and capturing sub-genotype level diversity. Because complete MOCV genome characterization is an expensive and labor-intensive task, it makes sense to prioritize samples for whole-genome sequencing by diversity triage screening. To meet this demand, we developed a novel assay for accurate MOCV detection and genotyping, and comprehensive sub-genotype qualification to the level of phylogenetic groups (PGs). The assay included a novel set of oligonucleotide primers and probes, and it was implemented using digital polymerase chain reaction (dPCR). It offers sensitive, specific, and accurate detection, genotyping (MOCV1-MOCV3), and PG qualification (PG1-6) of MOCV DNA from clinical samples. The novel dPCR assay is suitable for MOCV diversity triage screening and prioritization of samples for complete MOCV genome characterization. |
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| Keywords: | Molluscum contagiosum, Molluscum contagiosum virus, digital PCR, diversity screening, molecular diagnostics, sample prioritization triage |
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| Publication status: | Published |
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| Publication version: | Version of Record |
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| Year of publishing: | 2024 |
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| Number of pages: | str. 1-8 |
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| Numbering: | Vol. 329, article 114993 |
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| PID: | 20.500.12556/DiRROS-30039  |
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| UDC: | 577.2 |
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| ISSN on article: | 1879-0984 |
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| DOI: | 10.1016/j.jviromet.2024.114993 |
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| COBISS.SI-ID: | 201868291 |
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| Note: | Nasl. z nasl. zaslona;
Opis vira z dne 16. 7. 2024;
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| Publication date in DiRROS: | 12.06.2026 |
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| Views: | 88 |
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| Downloads: | 61 |
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