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Title:Enhancing surgical safety : microbiological air control in operating theatres at University medical centre Maribor
Authors:ID Gradišnik, Lidija (Author)
ID Bunc, Gorazd (Author)
ID Ravnik, Janez (Author)
ID Velnar, Tomaž (Author)
Files:.pdf PDF - Presentation file, download (275,75 KB)
MD5: 11F9145DAAA870E9A8BDF92F972B50A3
 
URL URL - Source URL, visit https://www.mdpi.com/2075-4418/14/10/1054
 
Language:English
Typology:1.01 - Original Scientific Article
Organization:Logo UKC LJ - Ljubljana University Medical Centre
Abstract:Background: the aim of the study was to assess microbiological air quality in operating theatres by determining the level of microbiological contamination of the air and critical surfaces using the passive air sampling method and compliance of the operating theatre staff with infection control measures. Materials and methods: The prospective study was conducted in the surgical block of the University Medical Centre Maribor. For two months continuously, ten operating theatres were assessed for microbial contamination of air and surfaces during quiet and active times of the day. A passive air sampling method with Petri dishes on an agar specially adapted for this purpose (plate count agar) was used. In addition, ten surgical procedures were observed to assess staff compliance with recommended practises. Results: Air samples met microbiological standards in all operating theatres. In both sampling sessions of the day (quiet and active periods), microbial contamination of the air was always within the limit of 10 CFU/m3 . The average number of bacterial colonies was zero to two during quiet phases and one to four during active phases. Approximately 60% of the isolates from the operating theatres belonged mainly to the genus Staphylococcus: S. epidermidis (36% of the isolates), S. hominis (17.5%) and S. haemolyticus (5.5%). The rest were identified as Streptococcus anginosus (23%) and Bacillus sp. (18%). Pathogenic bacteria and moulds were not present. In regard to staff compliance with good surgical practise, the former varied by behaviour and function, with non-compliance in pre-operative skin preparation and operating theatre congestion being notable. The cleanliness of the environment was satisfactory. Conclusions: Microbiological air control is extremely important for the safety and success of both surgical and postoperative practises. In spite of good results obtained in the study, further improvements in surgical staff compliance with good surgical practise are essential to reduce surgical site infections.
Keywords:surgical site infections, operating theatres, microbiological contamination, air control, good surgical practice
Publication status:Published
Publication version:Version of Record
Year of publishing:2024
Number of pages:str. [1]-13
Numbering:Vol. 14, iss. 10, art. 1054
PID:20.500.12556/DiRROS-29798 New window
UDC:502.3:613.15
ISSN on article:2075-4418
DOI:10.3390/diagnostics14101054 New window
COBISS.SI-ID:196142851 New window
Note:Nasl. z nasl. zaslona; Opis vira z dne 21. 5. 2024;
Publication date in DiRROS:05.06.2026
Views:120
Downloads:95
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Record is a part of a journal

Title:Diagnostics
Shortened title:Diagnostics
Publisher:MDPI AG
ISSN:2075-4418
COBISS.SI-ID:519963673 New window

Licences

License:CC BY 4.0, Creative Commons Attribution 4.0 International
Link:http://creativecommons.org/licenses/by/4.0/
Description:This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.

Secondary language

Language:Slovenian
Keywords:okužbe kirurškega mesta, operacijske dvorane, mikrobiološka kontaminacija, nadzor zraka, dobra kirurška praksa


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