| Title: | Computational modeling and characterization methods for rotating magnetic nanochain-enhanced lateral flow immunoassays |
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| Authors: | ID Orlov, Alexey V. (Author)
ID Malkerov, Juri A. (Author)
ID Rakitina, Alexandra S. (Author)
ID Kudriavtseva, Anastasiia (Author)
ID Minakov, Alexander A. (Author)
ID Tselikov, Daniil I. (Author)
ID Nikitin, Petr I. (Author)
ID Kralj, Slavko, Institut "Jožef Stefan" (Author) |
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| Files: |  URL - Source URL, visit https://www.sciencedirect.com/science/article/pii/S2215016126001548?via%3Dihub
 PDF - Presentation file, download (5,94 MB)
MD5: EB89A116154563A042131FF24683C16D
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| Language: | English |
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| Typology: | 1.01 - Original Scientific Article |
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| Organization: |  IJS - Jožef Stefan Institute
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| Abstract: | This article provides comprehensive methodological guidance for implementing rotating magnetic nanochain-enhanced lateral flow immunoassays with volumetric magnetic detection. Rotating magnetic nanochains act as microscale stirrers that substantially enhance antibody-antigen binding kinetics through convective mixing, yet their integration into lateral flow platforms presents unique technical challenges requiring both computational optimization and specialized characterization. We describe complete workflows for: (i) computational fluid dynamics modeling using COMSOL Multiphysics to simulate nanochain rotation, fluid flow, and mass transport enhancement; (ii) electron microscopy characterization of magnetic nanochain morphology and size distributions; (iii) rotating magnetic field generator design and operation; and (iv) magnetic particle quantification measurement procedures for volumetric signal readout. Each section provides step-by-step instructions with sufficient detail to enable independent replication. The described methods enable development of lateral flow assays achieving sub-nanogram detection limits with rapid (6-minute) analysis times, addressing critical needs in point-of-care diagnostics. These methods complement our related research article in Biosensors and Bioelectronics by providing the technical foundation necessary for adoption and adaptation of this technology by other laboratories. |
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| Keywords: | magnetic nanochains, lateral flow immunoassay, computational fluid dynamics, point-of-care diagnostic, rotating magnetic field, cardiac biomarkers |
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| Publication status: | Published |
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| Publication version: | Version of Record |
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| Submitted for review: | 12.03.2026 |
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| Article acceptance date: | 02.05.2026 |
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| Publication date: | 05.05.2026 |
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| Publisher: | Elsevier |
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| Year of publishing: | 2026 |
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| Number of pages: | str. 1-18 |
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| Numbering: | Vol. 16, [article no.] 103936 |
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| Source: | Nizozemska |
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| PID: | 20.500.12556/DiRROS-29485  |
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| UDC: | 620.3 |
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| ISSN on article: | 2215-0161 |
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| DOI: | 10.1016/j.mex.2026.103936 |
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| COBISS.SI-ID: | 278650115 |
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| Copyright: | © 2026 The Authors. |
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| Note: | Nasl. z nasl. zaslona;
Soavtor iz Slovenije: Slavko Kralj;
Opis vira z dne 19. 5. 2026;
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| Publication date in DiRROS: | 20.05.2026 |
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| Views: | 134 |
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| Downloads: | 91 |
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