| Title: | CRISPR/dCas9-based systems: mechanisms and applications in plant sciences |
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| Authors: | ID Soong Karlson, Chou Khai (Author)
ID Mohd-Noor, Siti Nurfadhlina (Author)
ID Nolte, Nadja Franziska (Author)
ID Tan, Boon Chin (Author) |
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| Files: |  URL - Source URL, visit https://doi.org/10.3390/plants10102055
 PDF - Presentation file, download (3,60 MB)
MD5: 49CB864B70A7FD21BD76BA0646C5A15B
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| Language: | English |
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| Typology: | 1.02 - Review Article |
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| Organization: |  NIB - National Institute of Biology
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| Abstract: | RNA-guided genomic transcriptional regulation tools, namely clustered regularly interspaced short palindromic repeats interference (CRISPRi) and CRISPR-mediated gene activation (CRISPRa), are a powerful technology for gene functional studies. Deriving from the CRISPR/Cas9 system, both systems consist of a catalytically dead Cas9 (dCas9), a transcriptional effector and a single guide RNA (sgRNA). This type of dCas9 is incapable to cleave DNA but retains its ability to specifically bind to DNA. The binding of the dCas9/sgRNA complex to a target gene results in transcriptional interference. The CRISPR/dCas9 system has been explored as a tool for transcriptional modulation and genome imaging. Despite its potential applications and benefits, the challenges and limitations faced by the CRISPR/dCas9 system include the off-target effects, protospacer adjacent motif (PAM) sequence requirements, efficient delivery methods and the CRISPR/dCas9-interfered crops being labeled as genetically modified organisms in several countries. This review highlights the progression of CRISPR/dCas9 technology as well as its applications and potential challenges in crop improvement. |
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| Keywords: | CRISPR interference, CRISPR/dCas9 system, crop improvement, gene silencing, RNAi, transcriptional regulation |
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| Publication status: | Published |
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| Publication version: | Version of Record |
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| Publication date: | 29.09.2021 |
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| Year of publishing: | 2021 |
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| Number of pages: | 1-22 str. |
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| Numbering: | Vol. 10, iss. 10, [article no.] 2055 |
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| PID: | 20.500.12556/DiRROS-28763  |
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| UDC: | 277.2 |
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| ISSN on article: | 2223-7747 |
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| DOI: | 10.3390/plants10102055 |
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| COBISS.SI-ID: | 273252867 |
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| Note: | Soavtorji: Siti Nurfadhlina Mohd-Noor, Nadja Nolte, Boon Chin Tan;
Nasl. z nasl. zaslona;
Opis vira z dne 26. 3. 2026;
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| Publication date in DiRROS: | 01.04.2026 |
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| Views: | 44 |
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| Downloads: | 19 |
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