| Title: | Absolute quantification of microRNA miR-875-5p in temporal artery biopsies and its biomarker potential for giant cell arteritis |
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| Authors: | ID Bolha, Luka (Author)
ID Smajlović, Elvisa (Author)
ID Hočevar, Alojzija (Author) |
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| Files: |  PDF - Presentation file, download (1,11 MB)
MD5: E8683A34D210C7059EA7FBA1D9579E8D
 URL - Source URL, visit https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2026.1676244/full
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| Language: | English |
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| Typology: | 1.03 - Other scientific articles |
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| Organization: |  UKC LJ - Ljubljana University Medical Centre
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| Abstract: | Objectives: To perform absolute quantification of miR-875-5p expression in temporal artery biopsies (TABs) of patients with giant cell arteritis (GCA), associate miR-875-5p copy number with histological and clinical characteristics of patients with GCA, and evaluate the diagnostic value of absolute copy number of miR-875-5p in GCA. Methods: The study included formalin-fixed, paraffin-embedded TABs of 45 treatment-naïve clinically proven patients with GCA, and 19 non-GCA controls. Of the included patients with GCA, 29 had histologically positive and 16 histologically negative TABs. Expression of miR-875-5p was assessed through utilization of quantitative real-time PCR (qPCR) and absolute quantification by digital PCR (dPCR). Results: We determined significantly higher absolute copy number of miR-875-5p in histologically positive TABs of patients with GCA, compared to histologically negative TABs of GCA and non-GCA patients, which significantly correlated with the majority of TAB histopathological parameters and several clinical characteristics of patients with GCA. Notably, we showed a good diagnostic performance of absolute copy number of miR-875-5p in discriminating patients and controls, depending on the extent of vessel wall inflammation and remodeling in affected temporal arteries. Conclusion: Our study revealed that absolute quantification of miR-875-5p expression holds the potential to serve as a supporting biomarker in assessing vessel wall inflammation and remodeling in patients with GCA. Moreover, our results indicate the applicability of absolute quantification by dPCR in detecting low-abundance miRNAs in GCA-affected arterial tissue, whose limiting amounts hinder the utilization of classical qPCR. |
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| Keywords: | biomarker, digital PCR, giant cell arteritis, microRNA, temporal artery biopsy |
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| Publication status: | Published |
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| Publication version: | Version of Record |
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| Year of publishing: | 2026 |
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| Number of pages: | str. 1-8 |
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| Numbering: | Vol. 17, iss [article no.] 1731784 |
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| PID: | 20.500.12556/DiRROS-28609  |
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| UDC: | 616-097 |
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| ISSN on article: | 1664-3224 |
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| DOI: | 10.3389/fimmu.2026.1676244 |
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| COBISS.SI-ID: | 270816515 |
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| Note: | Nasl. z nasl. zaslona;
Opis vira z dne 6. 3. 2026;
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| Publication date in DiRROS: | 25.03.2026 |
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| Views: | 26 |
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| Downloads: | 10 |
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