| Title: | Effect of male sex-category on gene expression in the m. longissimus dorsi (LD) and m. semispinalis capitis (SSC) : expression profiling by high throughput sequencing |
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| Authors: | ID Vrecl, Milka (Author)
ID Fazarinc, Gregor (Author)
ID Poklukar Žnidaršič, Klavdija (Author) |
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| Files: |  URL - Source URL, visit https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE183240
Description: podatki na voljo po objavi
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| Language: | English |
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| Typology: | 2.20 - Complete scientific database of research data |
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| Organization: |  KIS - Agricultural Institute of Slovenia
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| Abstract: | RNA- sequencing was performed to compare the effect of male sex category, i.e. entire males (EM), surgical castrates (SC), and immunocastrates (IC) on differential gene expression in LD and SSC muscles. Extracted RNA from 6 randomly selected animals of each sex category and muscle were pooled together and used for library preparation and sequencing. A total of 71 and 36 differentially expressed genes (DEGs) were detected in LD and SSC, respectively (|log2(fold change) >1| and -log10(q-value) <0.005). In LD, 36 DEGs were detected between EM and IC. 29 DEGs were detected between EM and SC and only 6 DEGs were detected when comparing IC and SC. In SSC, the number of DEGs was lower compared to LD. Only 8 DEGs were detected when either EM and IC or EM and SC were compared. The number of DEGs between IC and SC was higher for SCC than for LD (20 vs. 6). In LD, downregulated genes predominated in EM compared to either IC or SC and were mainly associated with skeletal muscle tissue development, skeletal cell differentiation, and negative regulation of biological/cellular processes. When comparing IC and SC, predominantly upregulated genes were observed, and the enriched GO terms were related to various biological processes, including regulation of fibrinolysis, apoptosis, energy homeostasis, fatty acid oxidation, and behaviour. In SSC, significantly enriched GO terms were found only for predominantly upregulated genes in IC compared to SC and were related to the regulation of energy homeostasis and fatty acid oxidation, response to hormones/stimuli, and regulation of cellular ketone metabolic process. |
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| Publication version: | Version of Record |
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| Place of publishing: | Bethesda |
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| Place of performance: | Bethesda |
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| Publisher: | Gene Expression Omnibus (GEO) |
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| Year of publishing: | 2024 |
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| Year of performance: | 2024 |
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| Number of pages: | 1 spletni vir |
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| PID: | 20.500.12556/DiRROS-28564  |
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| UDC: | 577.2 |
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| COBISS.SI-ID: | 230645251 |
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| Note: | Nasl. z nasl. zaslona;
Opis vira z dne 28. 3. 2025;
ǂBioProject ǂPRJNA759775;
GSE183240;
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| Publication date in DiRROS: | 24.03.2026 |
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| Views: | 259 |
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| Downloads: | 113 |
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| Metadata: |    |
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