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Title:Vpliv oljčnih tropin na mikrobno združbo tal : poročilo o ciljnoraziskovalnem projektu CRP V4-2216
Authors:ID Babič, Rok (Author)
ID Fantinič, Jakob (Author)
ID Bastida, Felipe (Author)
ID Podgornik, Maja (Author)
Files:URL URL - Source URL, visit https://www.zrs-kp.si/publikacija/vpli-oljcnih-tropin-na-mikrobiom/
 
.pdf PDF - Presentation file, download (4,54 MB)
MD5: 846330CB222554F5DFE0B4E15EC4F223
 
Language:Slovenian
Typology:2.12 - Final Research Report
Organization:Logo ZRS Koper - Science and Research Centre Koper
Abstract:V okviru projekta Go-ToS-Oil smo proučevali vpliv uporabe oljčnih tropin na sestavo mikrobne združbe in aktivnost talnih encimov v oljčniku sorte ‘Itrana’. Nanos tropin smo izvedli v različnih časovnih obdobjih, in sicer »1 teden«, »1 mesec«, »6 mesecev« in »1 leto« pred vzorčenjem, nato pa ugotavljali razlike v sestavi mikrobne združbe z uporabo analize metilnih estrov maščobnih kislin (FAMEs) ter sekvenciranjem regij 16S rRNA in ITS2. Analizirali smo tudi encimsko aktivnost ključnih talnih encimov: ureaze, alkalne fosfataze ter ß-glukozidaze, povezanih s kroženjem dušika, fosforja in ogljika. Dobljeni rezultati kažejo spremembe v sestavi mikrobne združbe in aktivnosti talnih encimov po nanosu mokrih oljčnih tropin. V zgodnjih fazah po uporabi smo opazili visoke vrednosti bakterijske biomase ob hkratni inhibiciji gliv, medtem ko se v poznejših fazah raznolikost in delež gliv nekoliko povečata. Nanos tropin je povzročil zmanjšanje vrstne bogatosti in prestrukturiranje mikrobne združbe. Ugotovitve glede vsebnosti talnih encimov kažejo, da uporaba tropin pomembno vpliva na mikrobno aktivnost.
Keywords:oljke, oljčne tropine, oljčniki, mikrobna združba, mikrobi, mikrobna aktivnost
Publication version:Version of Record
Place of publishing:Koper
Place of performance:Koper
Publisher:Annales ZRS
Year of publishing:2026
Year of performance:2026
Number of pages:1 spletni vir (1 datoteka PDF (21 str.))
PID:20.500.12556/DiRROS-28560 New window
ISBN:978-961-7276-11-4
UDC:633.852.73:631,4
DOI:10.35469/978-961-7276-11-4 New window
COBISS.SI-ID:272414723 New window
Copyright:Znanstveno-raziskovalno središče Koper
Note:Nasl. z nasl. zaslona; Opis vira z dne 24. 3. 2026;
Publication date in DiRROS:24.03.2026
Views:195
Downloads:126
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Document is financed by a project

Funder:ARIS - Slovenian Research and Innovation Agency
Project number:V4-2216
Name:Ohranjanje in izboljšanje proizvodnega potenciala tal za trajnostno pridelavo oljk (GO-TO S-OIL)

Licences

License:CC BY-NC-ND 4.0, Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
Link:http://creativecommons.org/licenses/by-nc-nd/4.0/
Description:The most restrictive Creative Commons license. This only allows people to download and share the work for no commercial gain and for no other purposes.
Licensing start date:24.03.2026

Secondary language

Language:English
Abstract:Within the GO-ToS-Oil project, we investigated the effects of olive pomace application on microbial community composition and soil enzyme activity in an ‘Itrana’ olive orchard. Olive pomace was applied at different intervals prior to sampling—1 week, 1 month, 6 months, and 1 year – and subsequent changes in the soil microbial community were assessed using fatty acid methyl ester (FAME) profiling and sequencing of the 16S rRNA and ITS2 regions. We also analysed the activity of key soil enzymes linked to nitrogen, phosphorus, and carbon cycling: urease, alkaline phosphatase, and β-glucosidase. The results show clear shifts in microbial community structure and soil enzyme activity following the application of fresh olive pomace. In the early stages after application, we observed increased bacterial biomass and fungal inhibition, while in later stages both fungal diversity and relative abundance increased. Application of olive pomace reduced species richness and led to a marked restructuring of the microbial community. Enzyme activity patterns further indicated that pomace application has a significant impact on soil microbial functioning.


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