| Title: | Effect of rearing systems and dietary protein levels on the skeletal muscle histomorphology and transcriptome profiles in indigenous Krškopolje Pig : expression profiling by high throughput sequencing |
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| Authors: | ID Vrecl, Milka (Author) ID Škrlep, Martin (Author) ID Poklukar Žnidaršič, Klavdija (Author) ID Fazarinc, Gregor (Author) ID Štrbenc, Malan (Author) ID Čandek-Potokar, Marjeta (Author) |
| Files: | URL - Source URL, visit https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE320088 Description: Podatki nedostopni, 20. 3. 2026, dostopni po 20. 2. 2030
PDF - Presentation file. (726,02 KB, This file will be accessible after 20.02.2030) MD5: 969957605D665B3927658E5566FBD90F
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| Language: | English |
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| Typology: | 2.20 - Complete scientific database of research data |
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| Organization: | KIS - Agricultural Institute of Slovenia
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| Abstract: | RNA-sequencing was performed to compare the effects of production systems (conventional indoor, vs. outdoor) and diets (standard protein, HP, vs. low protein, LP) within each rearing system on the transcriptomic profiles of two skeletal muscles, longissimus thoracis et lumborum (LL) and semispinalis capitis (SSC). Differentially expressed genes (DEGs) were identified with |log2FC| > 1 and q-value < 0.005. The effect of rearing system (indoor vs. outdoor) resulted in 354 DEGs in LL and 334 DEGs in SSC. The effect of diet within individual rearing system was less pronounced. In LL, HP vs. LP resulted in 23 DEGs under indoor and none under outdoor rearing system. In SSC, HP vs. LP resulted in 28 DEGs under indoor and 30 DEGs under outdoor rearing system. Pathway enrichment analysis revealed 42 pathways significantly enriched for HP vs. LP in the outdoor rearing system (across both muscles); additionally, 11 pathways were altered specifically in SSC for HP vs. LP under indoor rearing system, and 27 pathways were altered specifically in LL for HP vs. LP under outdoor rearing system |
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| Keywords: | RNA sequences, pigs, adipose tissue, high throughput sequencing, RNA, transcriptome profiles, gene expression, locel breeds, hybrid breeds, data, data set |
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| Publication status: | Published |
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| Publication version: | Version of Record |
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| Publication date: | 20.02.2026 |
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| Place of publishing: | Bethesda |
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| Place of performance: | Bethesda |
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| Publisher: | Gene Expression Omnibus (GEO) |
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| Year of publishing: | 2026 |
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| Year of performance: | 2026 |
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| Number of pages: | 1 spletni vir |
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| PID: | 20.500.12556/DiRROS-28453  |
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| UDC: | 636 |
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| COBISS.SI-ID: | 272369923  |
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| Note: | Nasl. z. nasl. zaslona;
Opis vira z dne 20. 3. 2026;
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| Publication date in DiRROS: | 20.03.2026 |
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| Views: | 244 |
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| Downloads: | 47 |
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