| Title: | Simple, fast, reliable: multiplex digital PCR quantification of 19 genetically modified soybean events |
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| Authors: | ID Jelenčič, Amadej (Author)
ID Štebih, Dejan (Author)
ID Demšar, Tina (Author)
ID Dobnik, David (Author) |
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| Files: |  URL - Source URL, visit https://www.tandfonline.com/doi/full/10.1080/21645698.2026.2635816#abstract
 PDF - Presentation file, download (1,03 MB)
MD5: 704A56AABA11400C1FC902A0D68FFF70
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| Language: | English |
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| Typology: | 1.01 - Original Scientific Article |
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| Organization: |  NIB - National Institute of Biology
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| Abstract: | Plant genetic engineering represents an important aspect of modern agriculture, and new geneticallymodified (GM) crop varieties are entering the market on a regular basis. This necessitates thedevelopment of high throughput multi-target analytical methods to detect and quantify theirpresence for regulatory compliance. In this study, we present a multiplex dPCR method for discrimi-native quantification of 19 GM soybean events and the lectin (Le1) endogene on a nanowell plate-based all-in-one dPCR system. The method consists of four 5-plex assays, taking advantage of theplatform’s multiple fluorescence detection channels. The assays complied with the minimum perfor-mance requirements in terms of specificity, trueness, precision, sensitivity and dynamic range,making them suitable for use in routine detection and quantification of GM crops. This methodrepresents the most comprehensive multi-target GM soybean quantification approach to date with-out the need for prior screening and features a simplified workflow, making it suitable for widespreadadoption. Our study sets a precedent for rapid and straightforward development of multiplex dPCRGM crop quantification assays to address the evolving demands of regulatory monitoring. |
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| Keywords: | genetically modified (GM) crops, transgenic, soybean, Glycine max (L.) merr, quantification, digital PCR (dPCR), multiplex, multi-target |
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| Publication status: | Published |
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| Publication version: | Version of Record |
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| Publication date: | 27.02.2026 |
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| Year of publishing: | 2026 |
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| Number of pages: | Str. 1-14 |
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| Numbering: | Vol. 17, no. 1, [article no.] 2635816 |
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| PID: | 20.500.12556/DiRROS-28436  |
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| UDC: | 577.2 |
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| ISSN on article: | 2164-5701 |
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| DOI: | 10.1080/21645698.2026.2635816 |
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| COBISS.SI-ID: | 270175747 |
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| Note: | Nasl. z nasl. zaslona;
Soavtorji: Dejan Štebih, Tina Demšar, David Dobnik;
Opis vira z dne 2. 3. 2026;
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| Publication date in DiRROS: | 19.03.2026 |
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| Views: | 220 |
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| Downloads: | 160 |
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