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Title:Izzivi uporabe visoko-pretočnega sekvenciranja (HTS) za rutinsko diagnostiko viroidov
Authors:ID Guček, Tanja (Author)
ID Radišek, Sebastjan (Author)
Files:URL URL - Source URL, visit https://www.dlib.si/details/URN:NBN:SI:spr-OS4I5CDJ
 
.pdf PDF - Presentation file, download (419,37 KB)
MD5: C1A4C45B8E179916AFE5A1A4940FE00A
 
Language:Slovenian
Typology:1.02 - Review Article
Organization:Logo IHPS - Slovenian Institute of Hop Research and Brewing
Abstract:Viroidne bolezni predstavljajo nenehno grožnjo svetovnemu kmetijstvu, saj lahko ti majhni in strukturno preprosti patogeni okužijo širok spekter gospodarsko pomembnih rastlin. Učinkovito preprečevanje in obvladovanje viroidnih okužb zato zahteva diagnostične pristope, ki omogočajo zanesljivo, občutljivo in specifično identifikacijo povzročiteljev bolezni. Z razvojem biokemije in molekularne biologije se je v zadnjih letih izboljšala občutljivost in specifičnost številnih diagnostičnih metod. Z zniževanjem stroškov sekvenciranja in večjo dostopnostjo se zelo hitro povečuje zanimanje za uporabo tehnologij visokopretočnega sekvenciranja (HTS) v rutinski rastlinski diagnostiki. Kljub velikemu potencialu HTS pa uvedba teh tehnologij v laboratorijsko prakso ostaja zahtevna. Med ključne izzive sodijo izbira ustrezne sekvenčne platforme in laboratorijskih protokolov, uporaba primernih bioinformacijskih orodij, zagotavljanje strokovno usposobljenega kadra ter vzpostavitev ustrezne računalniške infrastrukture. Čeprav so že oblikovani določeni standardi in smernice za uporabo HTS, ti zaradi specifičnih potreb laboratorijev niso univerzalno uporabni. Pregledni članek obravnava ključne vidike različnih pristopov HTS, njihove prednosti in omejitve ter prikazuje njihovo uporabnost na primeru diagnostike viroidnih bolezni.
Keywords:diagnostika, visoko-pretočno sekvenciranje (HTS), nanopore tehnologija (ONT), bioinformatika, viroidi
Publication date:19.12.2025
Year of publishing:2025
Number of pages:str. 72-84
Numbering:Št 32
PID:20.500.12556/DiRROS-27862 New window
UDC:632.38
ISSN on article:2536-1988
COBISS.SI-ID:262352387 New window
Copyright:Inštitut za hmeljarstvo in pivovarstvo Slovenije
Note:Nasl. z nasl. zaslona; Opis vira z dne 19. 12. 2025;
Publication date in DiRROS:26.02.2026
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Downloads:64
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Record is a part of a journal

Title:Hmeljarski bilten
Publisher:Inštitut za hmeljarstvo in pivovarstvo Slovenije (IHPS), = Slovenian Institute of Hop Research and Brewing (IHPS)
ISSN:2536-1988
COBISS.SI-ID:287262720 New window

Document is financed by a project

Funder:ARIS - Slovenian Research and Innovation Agency
Project number:P4-0077
Name:Kmetijske rastline - genetika in sodobne tehnologije

Licences

License:CC BY 4.0, Creative Commons Attribution 4.0 International
Link:http://creativecommons.org/licenses/by/4.0/
Description:This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
Licensing start date:01.01.2025

Secondary language

Language:English
Title:Challenges in the application of high-throughput sequencing for routine diagnostics of viroids
Abstract:Viroid diseases pose a continual threat to global agriculture, as these small and structurally simple pathogens are capable of infecting a wide range of economically important plant species. Effective prevention and management of viroid infections therefore require diagnostic approaches that ensure reliable, sensitive, and specific identification of the causal agents. Advances in biochemistry and molecular biology have, in recent years, significantly improved the sensitivity and specificity of many diagnostic methods. With decreasing sequencing costs and increasing accessibility, interest in the use of high-throughput sequencing (HTS) in routine plant diagnostics has grown rapidly. Despite the great potential of HTS, the implementation of these technologies into laboratory practice remains challenging. Key obstacles include the selection of an appropriate sequencing platform and laboratory protocols, the use of suitable bioinformatic tools, the availability of adequately trained personnel, and the establishment of suitable computational infrastructure. Although certain standards and guidelines for HTS have already been developed, they are not universally applicable due to the specific needs of individual laboratories. This review article discusses the key aspects of different HTS approaches, their advantages and limitations, and illustrates their applicability using the example of viroid disease diagnostics.
Keywords:diagnostics, high-throughput sequencing (HTS), Oxford Nanopore technology (ONT), bioinformatics, viroids


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  1. Hmeljarski bilten

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