| Title: | Advanced LC-MS/MS methodologies for the analysis of neuroactive compounds in tomato-based diets : insights from 2D in vitro digestion models |
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| Authors: | ID Kovačič, Ana, Institut "Jožef Stefan" (Author)
ID Masuero, Domenico (Author)
ID Garcia-Aloy, Mar (Author)
ID Mancini, Andrea (Author)
ID Vrhovšek, Urška (Author) |
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| Files: |  URL - Source URL, visit https://www.sciencedirect.com/science/article/pii/S2772753X26000146?via%3Dihub
 PDF - Presentation file, download (2,85 MB)
MD5: 6D31D06AA67A0650EE61BB80A58D1EAF
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| Language: | English |
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| Typology: | 1.01 - Original Scientific Article |
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| Organization: |  IJS - Jožef Stefan Institute
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| Abstract: | The digestive fate of neuroactive food compounds remains poorly understood despite growing interest in their role in brain health. This study developed advanced analytical methods to identify neuroprotective and neuro-disrupting compounds in tomatoes before and after digestion and following fecal fermentation. Using in vitro digestion and fermentation models, we characterized compound behavior during digestion. Most compounds were quantified in undigested tomatoes. Digestion revealed diverse outcomes: some compounds transformed (e.g., chlorogenic acids), others produced (e.g., linoleic acid), and several remained stable with potential to reach the colon (e.g., serotonin, rutin). Notably, neuro-disrupting compounds like bisphenols remained largely unaltered and bioaccessible in the colon. Fecal fermentation further showed dynamic changes, with some compounds decreasing (e.g., rutin) and others increasing (e.g., quercetin). These findings provide both methodological advances and mechanistic insights into neuroactive compound behavior during digestion, informing future research on gut-mediated dietary effects in the context of neurodegenerative disease prevention. |
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| Keywords: | neuroactive compounds, fecal fermentation, target analysis, bioaccesibility |
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| Publication status: | Published |
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| Publication version: | Version of Record |
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| Submitted for review: | 16.11.2025 |
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| Article acceptance date: | 20.01.2026 |
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| Publication date: | 20.01.2026 |
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| Publisher: | Elsevier |
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| Year of publishing: | 2026 |
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| Number of pages: | str. 1-25 |
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| Numbering: | Vol. , [article no.] 101231 |
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| Source: | Nizozemska |
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| PID: | 20.500.12556/DiRROS-27638  |
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| UDC: | 543 |
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| ISSN on article: | 2772-753X |
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| DOI: | 10.1016/j.focha.2026.101231 |
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| COBISS.SI-ID: | 266449923 |
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| Copyright: | © 2026 The Authors. |
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| Note: | Nasl. z nasl. zaslona;
Soavtorji iz Slovenije: Urška Vrhovšek;
Opis vira z dne 28. 1. 2026;
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| Publication date in DiRROS: | 17.02.2026 |
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| Views: | 217 |
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| Downloads: | 89 |
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