| Title: | Co-expression of holin gene improves heterologous cellulase secretion and surface display by lactic acid bacteria Lactococcus cremoris |
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| Authors: | ID Štravs, Petra, Institut "Jožef Stefan" (Author)
ID Repar, Lara, Institut "Jožef Stefan" (Author)
ID Fierobe, Henri-Pierre (Author)
ID Perret, Stéphanie (Author)
ID Berlec, Aleš, Institut "Jožef Stefan" (Author) |
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| Files: |  URL - Source URL, visit https://link.springer.com/article/10.1186/s13036-026-00625-0
 PDF - Presentation file, download (8,63 MB)
MD5: 21A6235F307514A47B719DB840E09D64
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| Language: | English |
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| Typology: | 1.01 - Original Scientific Article |
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| Organization: |  IJS - Jožef Stefan Institute
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| Abstract: | Genetically modified Lactococcus cremoris strains producing heterologous cellulases emerge as promising candidates for cost-effective bioprocessing of plant biowaste into high-value organic compounds. A key challenge in enabling efficient cellulose degradation by L. cremoris remains the effective secretion of heterologous cellulases. A potential solution would be to use genetic engineering to enhance the permeabilization of bacterial cell membrane and cell wall to facilitate the release of cellulases. This could be achieved by the co-expression of cellulase genes with genes encoding the prophage lytic proteins such as holin and endolysin. |
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| Keywords: | genetic engineering, lytic proteins, Lactococcus cremoris |
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| Publication status: | Published |
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| Publication version: | Version of Record |
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| Submitted for review: | 03.11.2025 |
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| Article acceptance date: | 12.01.2026 |
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| Publication date: | 12.02.2026 |
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| Publisher: | Springer Nature |
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| Year of publishing: | 2026 |
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| Number of pages: | str. 1-49 |
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| Numbering: | Vol. , iss. |
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| Source: | Švica |
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| PID: | 20.500.12556/DiRROS-27565  |
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| UDC: | 60 |
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| ISSN on article: | 1754-1611 |
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| DOI: | 10.1186/s13036-026-00625-0 |
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| COBISS.SI-ID: | 266410499 |
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| Copyright: | © The Author(s) 2026. |
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| Note: | Nasl. z nasl. zaslona;
Opis vira z dne 28. 1. 2026;
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| Publication date in DiRROS: | 13.02.2026 |
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| Views: | 295 |
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| Downloads: | 77 |
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| Metadata: |    |
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