| Title: | Previous short-term disuse dictates muscle geneexpression and physiological adaptations to subsequentresistance exercise |
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| Authors: | ID Franchi, Martino V. (Author)
ID Candia, Julián (Author)
ID Sarto, Fabio (Author)
ID Sirago, Giuseppe (Author)
ID Valli, Giacomo (Author)
ID Paganini, Matteo (Author)
ID Hartnell, Lisa M. (Author)
ID Giacomello, Emiliana (Author)
ID Toniolo, Luana (Author)
ID Monti, Elena (Author)
ID Nogara, Leonardo (Author)
ID Moro, Tatiana (Author)
ID Narici, Marco Vincenzo (Author) |
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| Files: |  PDF - Presentation file, download (4,75 MB)
MD5: 3ABC83FBD05E08468CE195E515D78351
 URL - Source URL, visit https://physoc.onlinelibrary.wiley.com/doi/10.1113/JP287003
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| Language: | English |
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| Typology: | 1.01 - Original Scientific Article |
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| Organization: |  ZRS Koper - Science and Research Centre Koper
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| Abstract: | Short-term unloading experienced following injury or hospitalisation induces muscle atrophy and weakness. The effects of exercise following unloading have been scarcely investigated. We investigated the functional and molecular adaptations to a resistance training (RT) programme following short-term unloading. Eleven males (22.09 ± 2.91 years) underwent 10 days of unilateral lower limb suspension (ULLS) followed by 21 days of knee extensor RT (three times/week). Data collection occurred at Baseline (LS0), after ULLS (LS10) and at active recovery (AR21). Knee extensor maximum voluntary contraction (MVC) was evaluated. Quadriceps volume was estimated by ultrasonography. Muscle fibre cross-sectional area, fibre type distribution, glycogen content and succinate dehydrogenase (SDH) activity were measured from vastus lateralis biopsies. Mitochondrial-related proteins were quantified by western blot and transcriptional responses were assessed by RNA sequencing. Following ULLS, quadriceps volume and MVC decreased significantly (3.7%, P < 0.05; 29.3%, P < 0.001). At AR21 (vs. LS10), MVC was fully restored (42%) and quadriceps volume increased markedly (18.6%, P < 0.001). Glycogen content and whole-body water increased at AR21 (14%, P < 0.001; 3.1%, P < 0.05). We observed a marked increase in fibre type I at AR21 (38%, P < 0.05). SDH immunoreactivity increased significantly after exercise (20%, P < 0.001). Mitochondrial fusion (MFN1, MFN2 and OPA1) and fission (DRP1) proteins were markedly increased by RT, and the most differentially expressed genes belonged to oxidative phosphorylation pathways. In contrast with what is usually observed after RT, oxidative metabolism, slow fibre type and mitochondrial dynamics were enhanced beyond expected. We propose that prior exposure to short-term muscle unloading may drive the nature of molecular adaptations to subsequent RT. |
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| Keywords: | exercise physiology, gene expression, muscle adaptation, muscle atrophy, musle physiology, muscle plasticity, resistance training, unloading responses |
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| Publication status: | Published |
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| Publication version: | Version of Record |
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| Publication date: | 01.01.2025 |
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| Year of publishing: | 2025 |
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| Number of pages: | str. 3725-3753 |
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| Numbering: | Vol. 603, iss. 13 |
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| PID: | 20.500.12556/DiRROS-25205  |
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| UDC: | 612 |
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| ISSN on article: | 0022-3751 |
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| DOI: | 10.1113/JP287003 |
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| COBISS.SI-ID: | 264568067 |
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| Copyright: | © 2025 The Author(s) |
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| Publication date in DiRROS: | 13.01.2026 |
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| Views: | 173 |
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| Downloads: | 104 |
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