| Title: | Functionality of potato virus Y coat protein in cell-to-cell movement dynamics is defined by its N-terminal region |
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| Authors: | ID Vozelj, Anže (Author)
ID Mahkovec Povalej, Tjaša (Author)
ID Stare, Katja (Author)
ID Tušek-Žnidarič, Magda (Author)
ID Bačnik, Katarina (Author)
ID Levak, Valentina (Author)
ID Gutiérrez-Aguirre, Ion (Author)
ID Podobnik, Marjetka (Author)
ID Gruden, Kristina (Author)
ID Coll Rius, Anna (Author)
ID Lukan, Tjaša (Author) |
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| Files: |  URL - Source URL, visit https://doi.org/10.1128/jvi.01400-25
 PDF - Presentation file, download (1,50 MB)
MD5: 976F9C5F819C4D96622A46E2D216CAA8
PDF - Presentation file, download (4,12 MB)
MD5: 61F8064D4BCA20EB9E93511D4E863A74
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| Language: | English |
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| Typology: | 1.01 - Original Scientific Article |
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| Organization: |  NIB - National Institute of Biology
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| Abstract: | Potato virus Y (PVY) is one of the top 10 economically most important plant viruses and responsible for major yield losses. We previously suggested the involvement of the N-terminal region of PVY coat protein (CP) in PVY spread. By constructing different N-terminal deletion mutants of the PVY N605 strain, we here show that deletions of 40 or more amino acid residues from the N-terminal region of the CP resulted in the PVY multiplication limited to primary infected cells in Nicotiana clevelandii plants. Deletion of 26 residues profoundly impaired PVY cell-to-cell movement and prevented systemic PVY spread, while deletions of 19-23 residues allowed delayed systemic PVY spread. Introduced point mutations in the identified region prevent (S21G) or delay (G20P) PVY movement. In summary, this work shows the significance of the CP N-terminus for movement of the PVY. |
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| Keywords: | potato virus Y, potato, coat proteins, viral movement, point mutations |
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| Publication status: | In print |
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| Publication version: | Author Accepted Manuscript |
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| Publication date: | 20.11.2025 |
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| Year of publishing: | 2025 |
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| PID: | 20.500.12556/DiRROS-24784  |
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| UDC: | 577.2 |
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| ISSN on article: | 1098-5514 |
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| DOI: | 10.1128/jvi.01400-25 |
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| COBISS.SI-ID: | 259503619 |
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| Note: | Nasl. z nasl. zaslona;
Soavtorji: Tjaša Mahkovec Povalej, Katja Stare, Magda Tušek Žnidarič, Katarina Bačnik, Valentina Levak, Ion Gutiérrez-Aguirre, Marjetka Podobnik, Kristina Gruden, Anna Coll, Tjaša Lukan;
Članek v PDF formatu obsega 14 str.;
Opis vira z dne 2. 12. 2025;
Online first: 20. Nov. 2025;
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| Publication date in DiRROS: | 18.12.2025 |
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| Views: | 30 |
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| Downloads: | 38 |
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