| Title: | MicroRNA expression profiles in sinonasal biopsies to support diagnosis of granulomatosis with polyangiitis |
|---|
| Authors: | ID Živanović, Milanka (Author)
ID Hočevar, Alojzija (Author)
ID Zidar, Nina (Author)
ID Volavšek, Metka (Author)
ID Bolha, Luka (Author) |
|---|
| Files: |  PDF - Presentation file, download (5,69 MB)
MD5: DD1554587C6335DCFAEBA7B8FAA53FAD
 URL - Source URL, visit https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2025.1579750/full
|
|---|
| Language: | English |
|---|
| Typology: | 1.01 - Original Scientific Article |
|---|
| Organization: |  UKC LJ - Ljubljana University Medical Centre
|
|---|
| Abstract: | Objectives: To identify aberrantly expressed microRNAs (miRNAs) in sinonasal tissue biopsies of patients with granulomatosis with polyangiitis (GPA), associate their expression profiles to sinonasal histopathology, and assess their differential expression between subgroups of clinically proven GPA patients, healthy controls and patients exhibiting inflammation of other etiology. Methods: We included formalin-fixed, paraffin-embedded biopsy tissue samples of sinonasal mucosa from 37 patients with clinically proven GPA, 15 patients with inflammation of other etiology and 14 control patients with normal histology. Of the included GPA patients, 20 patients had characteristic GPA-related histological features, while 17 patients displayed non-specific GPA histopathology in their sinonasal biopsy. Assessment of histological parameters was performed using histopathological techniques, and analysis of miRNA expression with miRCURY LNA miRNA miRNome Human PCR Panels and quantitative real-time PCR. Results: We determined expression of 306 miRNAs in sinonasal biopsy samples, which displayed different extent of dysregulation between individual patient groups. Based on their potential to discriminate between the controls, non-GPA and GPA patient subgroups, dysregulation of 11 miRNAs was further assessed, of which miR-1-3p/-21-3p/-93-5p/-155-5p/-1248/-31-3p/-182-5p/-183-5p and let-7b-5p held the potential to stratify patients based on their sinonasal tissue miRNA profile. Notably, several of these miRNAs were associated with the presence of granulomas, vasculitis and necrosis in sinonasal biopsies of GPA patients. Conclusion: Our study identifies novel miRNAs putatively implicated in the pathogenesis of GPA, and highlights dysregulated miRNAs as supporting biomarkers in establishing GPA diagnosis, particularly in the early phases of the disease, or in patients with atypical GPA presentation. |
|---|
| Keywords: | biomarker, granulomatosis with polyangiitis, inflammation, microRNA, sinonasal tissue |
|---|
| Publication status: | Published |
|---|
| Publication version: | Version of Record |
|---|
| Year of publishing: | 2025 |
|---|
| Number of pages: | str. 1-14 |
|---|
| Numbering: | Vol. 16 |
|---|
| PID: | 20.500.12556/DiRROS-24507  |
|---|
| UDC: | 616-002 |
|---|
| ISSN on article: | 1664-3224 |
|---|
| DOI: | 10.3389/fimmu.2025.1579750 |
|---|
| COBISS.SI-ID: | 234656003 |
|---|
| Note: | Nasl. z nasl. zaslona;
Opis vira z dne 30. 4. 2025;
|
|---|
| Publication date in DiRROS: | 03.12.2025 |
|---|
| Views: | 55 |
|---|
| Downloads: | 36 |
|---|
| Metadata: |    |
|---|
|
:
|
Copy citation |
|---|
| | | | Share: |  |
|---|
Hover the mouse pointer over a document title to show the abstract or click
on the title to get all document metadata. |
