| Title: | Comparison of self-collected and healthcare worker-collected rectovaginal swabs for group B streptococcus detection in pregnancy using PCR with a commercial collection-enrichment device |
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| Authors: | ID Kukovica, Iva (Author)
ID Omahen, Neža (Author)
ID Klobučar, Nika (Author)
ID Bučar, Martina (Author)
ID Franko Rutar, Anita (Author)
ID Perme, Tina (Author)
ID Lučovnik, Miha (Author)
ID Jeverica, Samo (Author) |
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| Files: |  PDF - Presentation file, download (214,14 KB)
MD5: 8AADB8C28CD0673D2274F5C99AE04AE7
 URL - Source URL, visit https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2025.1541319/full
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| Language: | English |
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| Typology: | 1.01 - Original Scientific Article |
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| Organization: |  UKC LJ - Ljubljana University Medical Centre
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| Abstract: | Introduction: Universal screening for the detection of group B streptococcus (GBS) colonization in pregnant women was recently introduced in Slovenia. The aim of our study was to determine whether self-collection of rectovaginal swabs is a valid alternative to collection by healthcare workers (HCWs). Methods: A prospective, multicenter study was conducted between June and November 2023. A total of 227 pregnant women (aged 20 to 44 years) from the University Medical Center Ljubljana (n = 136), the Novo mesto Community Health Center (n = 48) and the Trebnje Community Health Center (n = 43) were included. Two swabs were taken: swab A by the HCWs using standard semi-solid Amies transport medium (Meus; current standard) and swab B by the pregnant woman following visual instructions using a commercial LIM Broth (Copan). Swabs were inoculated onto ChromID Strepto B (STRB) agars directly and after overnight enrichment in LIM broth. The NeuMoDx GBS assay was performed from the enrichment broth. A self-assessment questionnaire was completed after sampling. Performance characteristics were calculated and compared between different diagnostics test algorithms using McNemar’s test for paired samples. Results: Overall, GBS was detected in 18% (95% CI 13–23%; n = 40) of swabs A and 19% (95% CI 14–25%; n = 43) of swabs B. PCR was superior in both groups. In the group of swabs collected by HCWs, 4 (40 vs. 36; 11.1% difference; p = 0.046) and 3 (40 vs. 37; 8.1% difference; p = 0.083) additional positives were detected with PCR compared to direct and enrichment culture, respectively; in the group of self-collected swabs, 4 (43 vs. 39; 10.3% difference; p = 0.046) and 6 (43 vs. 36; 16.2% difference; p = 0.014) additional positives were detected with PCR compared to direct and enrichment culture, respectively. Self-collection showed a trend towards a higher diagnostic yield. PCR after enrichment from self-collected samples was found to be the most. sensitive method overall. 58.5% (n = 124/212; 95% CI 52–65%) of women would prefer the swabs taken by HCWs. Discussion: Self-collection of rectovaginal swabs during pregnancy is a good alternative to HCW-collected swabs. PCR from enrichment broth was better for the detection of GBS compared to enrichment culture. Majority of women preferred swabs taken by HCWs. |
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| Keywords: | group B streptococcus, self-collection, enrichment culture, PCR, chromogenic agar, pregnancy, screening |
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| Publication status: | Published |
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| Publication version: | Version of Record |
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| Year of publishing: | 2025 |
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| Number of pages: | 8 str. |
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| Numbering: | Vol. 16, [article no.] 1541319 |
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| PID: | 20.500.12556/DiRROS-24478  |
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| UDC: | 618.1 |
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| ISSN on article: | 1664-302X |
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| DOI: | 10.3389/fmicb.2025.1541319 |
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| COBISS.SI-ID: | 225553667 |
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| Note: | Nasl. z nasl. zaslona;
Opis vira z dne 9. 2. 2025;
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| Publication date in DiRROS: | 01.12.2025 |
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| Views: | 89 |
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| Downloads: | 44 |
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