| Title: | Expression of inducible damage-associated molecular patterns after interleukin-12 gene electrotransfer in mouse melanoma and colorectal cell lines |
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| Authors: | ID Medved, Ajda (Author)
ID Omerzel, Maša (Author)
ID Jesenko, Tanja (Author)
ID Buček, Simon (Author)
ID Serša, Gregor (Author)
ID Čemažar, Maja (Author) |
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| Files: |  URL - Source URL, visit https://doi.org/10.1016/j.biopha.2025.118414
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| Language: | English |
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| Typology: | 1.01 - Original Scientific Article |
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| Organization: |  OI - Institute of Oncology
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| Abstract: | Interleukin-12 (IL-12) has demonstrated potent antitumor effects by activating natural killer (NK) and T cells, but its systemic administration poses challenges due to toxicity. This study investigated gene electrotransfer (GET) as a localized delivery method for IL-12 plasmids in B16F10 and CT26 tumor cells and its effect on the expression of damage-associated molecular patterns (DAMPs) and several cytokines. We observed the activation of cytosolic sensors and cytokine production, with the expression of IL-6 and TNF-α upregulated only after IL-12 GET, suggesting the involvement of inducible damage-associated molecular patterns (iDAMPs) in the immune response to IL-12 gene therapy. These findings highlight the multifaceted immune activation triggered by GET, offering insights for improving IL-12-based cancer therapies. |
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| Keywords: | gene electrotransfer, cytokine, interleukin-12 |
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| Publication status: | Published |
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| Publication version: | Version of Record |
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| Submitted for review: | 20.05.2025 |
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| Article acceptance date: | 29.07.2025 |
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| Publication date: | 01.08.2025 |
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| Publisher: | Elsevier Masson SAS |
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| Year of publishing: | 2025 |
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| Number of pages: | str. 118414-1-118414-15 |
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| Numbering: | Vol. 190, no. |
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| PID: | 20.500.12556/DiRROS-24410  |
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| UDC: | 602.6/.7 |
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| ISSN on article: | 1950-6007 |
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| DOI: | 10.1016/j.biopha.2025.118414 |
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| COBISS.SI-ID: | 246024963 |
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| Copyright: | by Authors |
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| Note: | Nasl. z nasl. zaslona;
Opis vira z dne 20. 8. 2025;
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| Publication date in DiRROS: | 05.12.2025 |
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| Views: | 107 |
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| Downloads: | 20 |
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