| Title: | Circulating miRNAs as potential non-invasive biomarkers for ANCA-associated glomerulonephritis |
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| Authors: | ID Bošnjak, Matic (Author)
ID Večerić-Haler, Željka (Author)
ID Pipan Tkalec, Živa (Author)
ID Tomšič, Jakob (Author)
ID Boštjančič, Emanuela (Author)
ID Kojc, Nika (Author) |
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| Files: |  PDF - Presentation file, download (2,00 MB)
MD5: 6AC47B2CB420D34F9ECF3504325B024C
 URL - Source URL, visit https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2025.1599043/full
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| Language: | English |
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| Typology: | 1.01 - Original Scientific Article |
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| Organization: |  UKC LJ - Ljubljana University Medical Centre
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| Abstract: | Introduction: Anti-neutrophil cytoplasmic antibody (ANCA)-associated vasculitis (AAV) is characterized by necrotizing small vessel vasculitis that frequently manifests as glomerulonephritis (AAV-GN). An accurate noninvasive biomarker reflecting active AAV-GN remains elusive. The knowledge of microRNAs (miRNAs), which have been considered as disease-specific biomarkers, is scarce and lacks validated data in AAV. Methods: This study validated a renal tissue expression profile of candidate miRNAs specific to AAV-GN selected through prior screening using independent cohorts. The analysis was extended to serum samples to explore the potential of a circulating miRNA panel as a noninvasive biomarker for active AAV-GN. To substantiate the findings, we correlated the molecular data with clinical and histologic markers of AAV-GN activity. Results: We identified miR-21-3p, miR-181a-5p, and miR-181d-5p as potential biomarkers distinguishing AAV-GN from non-AAV renal diseases and healthy controls. In addition, miR-21-3p and miR-181d-5p correlated with the presence of active AAV-GN, while miR-181a-5p differentiated AAV-GN subtypes based on ANCA antigen specificity. ROC curve analysis demonstrated that the combined serum expression of miR-21-3p and miR-181a-5p reliably distinguished AAV-GN from other renal pathologies, including ANCA-positive cases without histologic evidence of AAV-GN. Conclusion: Our findings highlight the potential of circulating miRNA expression signature as a noninvasive biomarker for ongoing AAV-GN in the appropriate setting. Larger confirmatory studies are essential to support the clinical application of miRNA-based biomarkers in AAV-GN diagnostics and disease monitoring. |
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| Keywords: | ANCA, biomarker, glomerulonephritis, microRNA, vasculitis, epigenetics |
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| Publication status: | Published |
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| Publication version: | Version of Record |
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| Year of publishing: | 2025 |
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| Number of pages: | str. 1-12 |
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| Numbering: | Vol. 16, [Article no.] 1599043 |
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| PID: | 20.500.12556/DiRROS-24387  |
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| UDC: | 616-097 |
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| ISSN on article: | 1664-3224 |
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| DOI: | 10.3389/fimmu.2025.1599043 |
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| COBISS.SI-ID: | 246506499 |
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| Note: | Nasl. z nasl. zaslona;
Opis vira z dne 25. 8. 2025;
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| Publication date in DiRROS: | 26.11.2025 |
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| Views: | 95 |
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| Downloads: | 68 |
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