| Title: | Multiplex quantification of 19 GM soybean lines using digital PCR |
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| Authors: | ID Jelenčič, Amadej (Author)
ID Štebih, Dejan (Author)
ID Demšar, Tina (Author)
ID Dobnik, David (Author) |
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| Files: |  URL - Source URL, visit https://doi.org/10.1007/978-1-0716-4767-7_2
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| Language: | English |
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| Typology: | 1.16 - Independent Scientific Component Part or a Chapter in a Monograph |
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| Organization: |  NIB - National Institute of Biology
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| Abstract: | The European Union (EU) imposes strict regulations on the presence of genetically modified (GM) material in food and feed, requiring thorough testing of samples for various GM lines. Although traditional quantitative real-time PCR (qPCR) methods are sensitive and robust, they are not cost-effective for managing large numbers of GM events due to their limited multiplexing capabilities. Conversely, digital PCR (dPCR) is capable of robust quantitative multiplexing in addition to other benefits such as absolute quantification and better tolerance of PCR inhibitors. In this context, we present a protocol for multiplex quantification of 19 GM soybean lines using dPCR as an improvement over the currently used simplex qPCR approach. This method enables simple and robust quantification of common GM soybean lines with a relatively low number of reactions. |
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| Keywords: | genetically modified soybean, GMO, multiplexing, food and feed, food regulation, digital PCR (dPCR) |
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| Publication status: | Published |
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| Publication version: | Author Accepted Manuscript |
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| Year of publishing: | 2026 |
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| Number of pages: | Str. 11-27 |
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| PID: | 20.500.12556/DiRROS-24370  |
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| UDC: | 577.2 |
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| DOI: | 10.1007/978-1-0716-4767-7_2 |
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| COBISS.SI-ID: | 254682371 |
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| Note: | Nasl. z nasl. zaslona;
Soavtorji: Dejan Štebih, Tina Demšar, and David Dobnik;
Opis vira z dne 24. 10. 2025;
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| Publication date in DiRROS: | 26.11.2025 |
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| Views: | 89 |
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| Downloads: | 19 |
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