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Title:Sampling and identification of microplastics in groundwater
Authors:ID Kovač Viršek, Manca (Author)
ID Bizjak, Tine (Author)
ID Bizjak, Nejc (Author)
ID Mali, Nina (Author)
Files:URL URL - Source URL, visit https://dx.doi.org/10.3791/68652-v
 
.pdf PDF - Presentation file, download (1,09 MB)
MD5: 5929888F7A1976E2254CA20F8C1CAF29
 
Language:English
Typology:1.01 - Original Scientific Article
Organization:Logo NIB - National Institute of Biology
Abstract:Microplastics pollution in groundwater remains significantly underreported within scientific literature. This paper presents a comprehensive protocol outlining the methodology for the sampling of groundwater from boreholes, as well as the steps of microplastics separation and analysis. It provides an extensive description of a filtration sampling system designed specifically for this purpose, along with the detailed sampling procedure. In addition, it presents the laboratory analysis of microplastic particles, including their characterization based on size, shape, color, transparency, and chemical structure using attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR) and micro-FTIR spectroscopy. Factors that can influence results are discussed, and special attention is paid to preventing contamination of samples. The methodology described also considers the requirements of the Annex of Commission Delegated Decision (EU) 2024/1441 of 11 March 2024, supplementing Directive (EU) 2020/2184 of the European Parliament and of the Council. This comprehensive written protocol, accompanied by video guidance, is intended to support the development of a synchronized methodology for monitoring microplastics in groundwater or drinking water. This resource will be of interest to researchers in the field of microplastics worldwide.
Keywords:microplastics, groundwater, spectroscopic methods, pollution
Publication status:Published
Publication version:Version of Record
Publication date:07.11.2025
Year of publishing:2025
Number of pages:str. 1-19
Numbering:Vol. 225, [art. no.] ǂe68652
PID:20.500.12556/DiRROS-24314 New window
UDC:556.3
ISSN on article:1940-087X
DOI:10.3791/68652 New window
COBISS.SI-ID:257986563 New window
Note:Nasl. z nasl. zaslona; Opis vira z dne 20. 11. 2025;
Publication date in DiRROS:26.11.2025
Views:445
Downloads:156
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Record is a part of a journal

Title:Journal of visualized experiments
Shortened title:J. vis. exp.
Publisher:MYJoVE Corporation
ISSN:1940-087X
COBISS.SI-ID:6867284 New window

Document is financed by a project

Funder:ARIS - Slovenian Research and Innovation Agency
Project number:J1-50030-2023
Name:Izboljšane metode za ugotavljanje transportnih procesov mikroplastike v virih podzemne vode (GWMicroPlast)

Funder:ARIS - Slovenian Research and Innovation Agency
Project number:P1-0020-2020
Name:Podzemne vode in geokemija

Licences

License:CC BY-NC-ND 3.0, Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported
Link:http://creativecommons.org/licenses/by-nc-nd/3.0/
Description:You are free to reproduce and redistribute the material in any medium or format. You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use. You may not use the material for commercial purposes. If you remix, transform, or build upon the material, you may not distribute the modified material. You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.

Secondary language

Language:Slovenian
Keywords:mikroplastika, podtalnica, spektroskopske metode, onesnaženje


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