| Title: | A rapid method for determination of rosuvastatin in blood plasma with supported liquid extraction |
|---|
| Authors: | ID Dermota, Tjaša (Author)
ID Božič Mijovski, Mojca (Author)
ID Trontelj, Jurij (Author) |
|---|
| Files: |  PDF - Presentation file, download (2,28 MB)
MD5: E2C709D800C13807E371713873D48E38
 URL - Source URL, visit https://www.sciencedirect.com/science/article/pii/S2667145X25000094
|
|---|
| Language: | English |
|---|
| Typology: | 1.01 - Original Scientific Article |
|---|
| Organization: |  UKC LJ - Ljubljana University Medical Centre
|
|---|
| Abstract: | Introduction: Accurate measurement of rosuvastatin in plasma is critical for effective patient management and treatment monitoring following myocardial infarction (MI). Expensive solid-phase extraction (SPE) and timeconsuming liquid-liquid extraction (LLE) have been established for quantifying rosuvastatin. Supported liquid extraction (SLE) could offer a rapid, cost-effective alternative. Objectives: This study aimed to develop and validate a rapid, cost-effective, accurate, and precise method for quantifying rosuvastatin in high-dose plasma samples from patients following MI. Methods: Rosuvastatin was extracted from EDTA plasma using SLE and quantified with LC-MS/MS with positive electrospray ionization. The method was validated according to ICH M10 guidelines, focusing on selectivity, matrix effect, accuracy, precision, linearity, and carryover. Rosuvastatin-D6 was used as an internal standard. Additionally, thirty plasma samples from patients on high-dose rosuvastatin therapy (20 or 40 mg/day) following MI were analyzed by both LLE and SLE methods and compared. Results: The method was successfully validated, demonstrating linearity across a range of 0.1 ng/mL to 50 ng/mL. Compared to the LLE method, SLE achieved superior extraction recovery (96.3 % vs. 60 %) and precision (RSD: 11.9 % vs. 13.6 %) at 0.3 ng/mL rosuvastatin, with a lower absolute matrix effect (12.7 % vs.-36.7 %). Accuracy was comparable (109.3 % vs. 92.8 %). Although SLE involves higher initial costs, it significantly enhances throughput, reduces solvent usage, and minimizes contamination and equipment wear. Conclusion: This study validates SLE as a superior method for quantifying rosuvastatin in plasma, outperforming LLE in recovery, reproducibility, and automation. SLE offers greater accuracy and reliability, making it ideal for high-throughput applications. |
|---|
| Keywords: | supported liquid extraction, liquid-liquid extraction, rosuvastatin, LC-MS/MS, myocardial infarction, solid-phase extraction, blood plasma |
|---|
| Publication status: | Published |
|---|
| Publication version: | Version of Record |
|---|
| Year of publishing: | 2025 |
|---|
| Number of pages: | str. 29-36 |
|---|
| Numbering: | Vol. 36 |
|---|
| PID: | 20.500.12556/DiRROS-24301  |
|---|
| UDC: | 543.544:61 |
|---|
| ISSN on article: | 2667-145X |
|---|
| DOI: | 10.1016/j.jmsacl.2025.04.003 |
|---|
| COBISS.SI-ID: | 245998595 |
|---|
| Note: | Nasl. z nasl. zaslona;
Opis vira z dne 20. 8. 2025;
|
|---|
| Publication date in DiRROS: | 24.11.2025 |
|---|
| Views: | 162 |
|---|
| Downloads: | 61 |
|---|
| Metadata: |    |
|---|
|
:
|
Copy citation |
|---|
| | | | Share: |  |
|---|
Hover the mouse pointer over a document title to show the abstract or click
on the title to get all document metadata. |
