| Title: | Transcriptomics‑based analysis of acetate and propionate transport and metabolism in Yarrowia lipolytica |
|---|
| Authors: | ID Žganjar, Mia, Institut "Jožef Stefan" (Author)
ID Sanz-Mata, David (Author)
ID Hancman, Urška (Author)
ID Čadež, Neža (Author)
ID Gostinčar, Cene (Author)
ID Gonzales-Fernandez, Cristina (Author)
ID Tomás Pejó, Elia (Author)
ID Petrovič, Uroš, Institut "Jožef Stefan" (Author) |
|---|
| Files: |  URL - Source URL, visit https://biotechnologyforbiofuels.biomedcentral.com/articles/10.1186/s13068-025-02713-7
 PDF - Presentation file, download (3,97 MB)
MD5: FEE120542D7E2F2923F94C33CD218BE5
Description: The assembled genome and raw expression data have been deposited at GeneBank under the BioProject accession number [PRJNA955139](https:/www.ncbi.nlm.nih.gov/bioproject/?term=PRJNA955139).
|
|---|
| Language: | English |
|---|
| Typology: | 1.01 - Original Scientific Article |
|---|
| Organization: |  IJS - Jožef Stefan Institute
|
|---|
| Abstract: | Background. Yarrowia lipolytica is a promising host for sustainable microbial oil production from waste-derived carboxylic acids such as acetate and propionate. Nonetheless, the molecular mechanisms underlying the metabolism and assimilation of these substrates, particularly under nitrogen limitation, are still not fully understood. Results. We conducted a multi-condition transcriptomic analysis of Y. lipolytica strain EXF-17398 under nitrogen-limiting conditions to investigate its transcriptional adaptation to acetate and propionate utilisation. Our results revealed distinct transcriptional responses associated with metabolic adaptation, including the coordinated regulation of Jen and Gpr carboxylate transporter families, suggesting a dual system for carboxylate uptake. JEN5 and GPR1 appear central to propionate and acetate utilisation, respectively. Our data suggest that propionate toxicity is mitigated through its conversion via the methylcitrate cycle and potentially the malonate semialdehyde pathway, preventing accumulation of cytotoxic propionyl-CoA in the cytosol. The upregulation of carnitine acyltransferases suggests active mitochondrial transport of acyl-CoAs, linking detoxification with energy metabolism. Under tested conditions, the de novo lipid synthesis was consistent with carbon overflow from acetyl-CoA and propionyl-CoA, supported by intracellular nitrogen recycling and redox balancing, independent of classical nitrogen regulatory pathways. Conclusions. These findings illustrate the capacity of Y. lipolytica to coordinate carbon and nitrogen metabolism during carboxylate utilisation, such as acetate and propionate, offering insights to guide the optimisation of microbial oil production from renewable feedstocks. |
|---|
| Keywords: | propionate, transport, metabolism |
|---|
| Publication status: | Published |
|---|
| Publication version: | Version of Record |
|---|
| Submitted for review: | 17.06.2025 |
|---|
| Article acceptance date: | 19.10.2025 |
|---|
| Publication date: | 10.11.2025 |
|---|
| Publisher: | Springer Nature |
|---|
| Year of publishing: | 2025 |
|---|
| Number of pages: | str. 1-17 |
|---|
| Numbering: | Vol. 18, article no. 112 |
|---|
| Source: | Švica |
|---|
| PID: | 20.500.12556/DiRROS-24229  |
|---|
| UDC: | 57 |
|---|
| ISSN on article: | 2731-3654 |
|---|
| DOI: | 10.1186/s13068-025-02713-7 |
|---|
| COBISS.SI-ID: | 257066755 |
|---|
| Copyright: | © The Author(s) 2025. |
|---|
| Note: | Nasl. z nasl. zaslona;
Soavtorji iz Slovenije: Urška Hancman, Neža Čadež, Cene Gostinčar, Uroš Petrovič;
Opis vira z dne 13. 11. 2025;
|
|---|
| Publication date in DiRROS: | 21.11.2025 |
|---|
| Views: | 167 |
|---|
| Downloads: | 91 |
|---|
| Metadata: |    |
|---|
|
:
|
Copy citation |
|---|
| | | | Share: |  |
|---|
Hover the mouse pointer over a document title to show the abstract or click
on the title to get all document metadata. |
