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Title:Chloroplast redox state changes indicate cell-to-cell signalling during the hypersensitive response : version v3
Authors:ID Lukan, Tjaša (Author)
ID Gruden, Kristina (Author)
ID Županič, Anže (Author)
ID Mahkovec Povalej, Tjaša (Author)
Files:URL URL - Similar work, visit https://doi.org/10.1111/nph.18425
 
URL URL - Source URL, visit https://doi.org/10.5281/zenodo.6417635
 
Language:English
Typology:2.20 - Complete scientific database of research data
Organization:Logo NIB - National Institute of Biology
Abstract:We performed detailed spatiotemporal analysis of chloroplast redox response to potato virus Y (PVY) infection in resistant Ny‐1-gene-bearing potato and its transgenic counterpart with impaired SA accumulation and compromised resistance. We found that the chloroplasts are highly oxidized in the cells adjacent to the cell death zone at different stages after virus inoculation in both genotypes. This hypothesis is further supported by highly induced formation of stroma filled tubules that extend from chloroplasts (stromules) in the cells adjacent to signalling cells. This dataset s a deposit of all the raw microscopy images of the study, plus the relevant metadata in ISA-tab compliant folder structure. After receiving reviews, we have made an additional experiment using a ROS inhibitor. The raw and processed data for this is in a separate file: _S_chlROS_inhibitor.zip
Keywords:cell signalling, chloroplasts, microscopy, ISA-tab, potato virus Y, redox responses, cell death, spatiotemporal analysis
Publication status:Published
Publication version:Version of Record
Place of publishing:Genève
Place of performance:Genève
Publisher:Zenodo
Year of publishing:2021
Year of performance:2021
Number of pages:1 spletni vir
PID:20.500.12556/DiRROS-23937 New window
UDC:577.2
DOI:10.5281/zenodo.6417635 New window
COBISS.SI-ID:245652995 New window
Note:Nasl. z. nasl. zaslona; Opis vira z dne 14. 8. 2025; Soavtorji: Gruden, Kristina; Županič, Anže; Mahkovec Povalej, Tjaša;
Publication date in DiRROS:24.10.2025
Views:258
Downloads:85
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Document is financed by a project

Funder:ARIS - Slovenian Research and Innovation Agency
Project number:P4-0165-2015
Name:Biotehnologija in sistemska biologija rastlin

Funder:ARIS - Slovenian Research and Innovation Agency
Project number:J4-7636-2016
Name:Prostorsko časovna analiza hipersenzitivnega odziva krompirja na krompirjev virus Y

Funder:ARIS - Slovenian Research and Innovation Agency
Project number:J4-1777-2019
Name:Odkrivanje mehanizmov učinkovitosti in specifičnosti imunske signalizacije v krompirju z inovativnim pridobivanjem podatkov in modeliranjem

Funder:ARIS - Slovenian Research and Innovation Agency
Project number:N4-0199-2021
Name:Vzpostavitev metodologij za študij imunosti krompirja na nivoju posameznih celic

Funder:ARIS - Slovenian Research and Innovation Agency
Project number:Z4-3217-2021
Name:Medsebojna povezanost z redoks stanjem posredovane signalizacije pri odpornosti krompirja na viruse

Funder:EC - European Commission
Project number:862858
Name:Accelerated Development of multiple-stress tolerAnt PoTato
Acronym:ADAPT

Licences

License:CC BY 4.0, Creative Commons Attribution 4.0 International
Link:http://creativecommons.org/licenses/by/4.0/
Description:This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.

Secondary language

Language:Slovenian
Keywords:krompir, krompirjev virus Y, stromule, analize slik, mikroskopija, celična smrt, prostorsko-časovne analize


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