| Title: | Deregulations of DNA damage-responsive genes, genes involved in the endocrine system, in an advanced in vitro 3D zebrafish hepatic cell model after exposure to Bisphenol A (BPA) and its emerging alternatives BPAF, BPAP and BPPH : version v1 |
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| Authors: | ID Štern, Alja (Author)
ID Rozman, Špela (Author)
ID Žegura, Bojana (Author) |
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| Files: |  URL - Source URL, visit https://doi.org/10.5281/zenodo.17144174
URL - Source URL, visit https://doi.org/10.1016/j.scitotenv.2025.180527
 PDF - Presentation file, download (1,09 MB)
MD5: F76F1903AC44775B428A91A93E3F6F45
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| Language: | English |
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| Typology: | 2.20 - Complete scientific database of research data |
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| Organization: |  NIB - National Institute of Biology
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| Abstract: | Bisphenol AF (BPAF), Bisphenol AP (BPAP), and Bisphenol PH (BPPH) are being introduced into consumer products to replace BPA and are subsequently detected in ecosystems. This study investigates the genotoxic and endocrine-disruptive potential of these emerging bisphenols using a 3D in vitro liver spheroid model derived from Danio rerio (ZFL cell line), on the transcriptional level. The selected genes targeted DNA damage response pathways (TP53, NER, BER) and endocrine-related signalling (Table 1). ZFL spheroids were prepared by a force floating method as described by Štampar et al. (2019)1. Four-day-old ZFL spheroids were exposed to BPA (50 and 200 µM), BPAF (25 and 100 µM), BPAP (25 and 100 µM), and BPPH (10 and 50 µM) for 24 (Table 2) and 96 (Table 3) hours. Following exposure, total RNA was extracted using the RNeasy® Mini Kit (Qiagen, Germany). RNA quality and quantity were assessed prior to reverse transcription (Applied Biosystems, USA), followed by gene-specific preamplification (TATAA PreAmp GrandMasterMix, Tataa Biocenter, Sweden). Gene expression analysis was performed using TaqMan Gene Expression Assays (Applied Biosystems, USA) on the Fluidigm One 48.48 Dynamic Array IFC microfluidic system as described by Štern et al. (2024)2. The generated data was analysed using the Fluidigm Gene Expression Analysis Software and with a free-accessible web program, quantGenious3. The difference in gene expression greater than 1.5-fold was considered a biologically important up/downregulation (relative expression >1.5 or <0.66, respectively). Statistically significant differences were analysed using ANOVA and Dunnett’s multiple comparison test in GraphPad Prism v9 (GraphPad Software, San Diego, CA, USA). |
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| Keywords: | bisphenols, genotoxicity, endocrine disruption, ZFL spheroids, gene expression, data |
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| Publication status: | Published |
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| Publication version: | Version of Record |
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| Publication date: | 17.09.2025 |
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| Place of publishing: | Genève |
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| Place of performance: | Genève |
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| Publisher: | Zenodo |
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| Year of publishing: | 2025 |
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| Year of performance: | 2025 |
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| Number of pages: | 1 spletni vir |
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| PID: | 20.500.12556/DiRROS-23749  |
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| UDC: | 615.9 |
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| DOI: | 10.5281/zenodo.17144174 |
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| COBISS.SI-ID: | 251043843 |
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| Note: | Nasl. z. nasl. zaslona;
Opis vira z dne 30. 9. 2025;
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| Publication date in DiRROS: | 30.09.2025 |
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| Views: | 265 |
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| Downloads: | 162 |
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