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Title:“Paste test” – a method for evaluating the fungal inhibition potential of ground wood samples
Authors:ID Keržič, Eli (Author)
ID Humar, Miha (Author)
Files:.pdf PDF - Presentation file, download (2,45 MB)
MD5: 5B016CC4127D71BC5DBF65DBEF2CCAC6
 
Language:English
Typology:1.01 - Original Scientific Article
Organization:Logo SciVie - Slovenian Forestry Institute
Abstract:As part of the »ULTRA – University of Ljubljana for a Sustainable Society« project, a new test method was introduced into the curriculum to provide insight into key properties that affect wood durability. This study aimed to adapt and verify the “paste test”, which uses wood dust in nutrient media to assess the inhibitory effect of wood components on fungal growth. The method was adapted to match the available laboratory equipment and simplified for educational use by students at the Department of Wood Science and Technology (Biotechnical Faculty, University of Ljubljana, Slovenia). The study involved testing fungal growth on nutrient media supplemented with spruce wood dust, with the goal of evaluating different grinding methods, nutrient media, and data analysis. Potato dextrose agar nutrient media was confirmed as a suitable alternative to malt extract agar. The wood dust fraction size had no significant effect on fungal inhibition, enabling the use of coarser, less time-saving grinding methods. Image analysis and logistic growth modelling were conducted using freely available software (ImageJ and R-Commander), which proved to be effective alternatives to proprietary tools. Visualization of growth using logistic growth rates offered a clearer comparative analysis, despite some loss of dynamic growth information.
Keywords:inhibition, wood chemistry, nutrient media, white rot fungi, brown rot fungi, logistic growth rate
Publication status:Published
Publication version:Version of Record
Publication date:01.01.2025
Year of publishing:2025
Number of pages:str. 37-48
Numbering:Vol. 136
PID:20.500.12556/DiRROS-22990 New window
UDC:630*84
ISSN on article:2335-3112
DOI:10.20315/ASetL.136.3 New window
COBISS.SI-ID:242542083 New window
Publication date in DiRROS:15.07.2025
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Downloads:235
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Record is a part of a journal

Title:Acta Silvae et Ligni
Publisher:Gozdarski inštitut Slovenije, založba Silva Slovenica, Biotehniška fakulteta, Oddelek za gozdarstvo in obnovljive gozdne vire, Biotehniška fakulteta, Oddelek za lesarstvo
ISSN:2335-3112
COBISS.SI-ID:266761216 New window

Document is financed by a project

Funder:Other - Other funder or multiple funders
Funding programme:European Union - NextGenerationEU
Project number:ULTRA
Name:UL za trajnostno družbo - ULTRA
Acronym:ULTRA

Funder:ARIS - Slovenian Research and Innovation Agency
Project number:P4-0015-2020
Name:Les in lignocelulozni kompoziti

Funder:ARIS - Slovenian Research and Innovation Agency
Project number:J7-50231-2024
Name:GROWTH: Rastni potencial in lastnosti lesa izbranih drevesnih vrst različnih provenienc: možnosti zaščite z modifikacijo in izzivi pri odzivanju na podnebne spremembe

Funder:ARIS - Slovenian Research and Innovation Agency
Project number:0481-09
Name:IC za pripravo, staranje in terensko testiranje lesa ter lignoceluloznih materialov
Acronym:OC LES PST

Licences

License:CC BY-SA 4.0, Creative Commons Attribution-ShareAlike 4.0 International
Link:http://creativecommons.org/licenses/by-sa/4.0/
Description:This Creative Commons license is very similar to the regular Attribution license, but requires the release of all derivative works under this same license.

Secondary language

Language:Slovenian
Title:Paste test
Abstract:V okviru projekta »ULTRA – Univerza v Ljubljani za trajnostno družbo« smo želeli v učni program uvesti nov način testiranja, da bi nam omogočil vpogled v izbrane lastnosti, ki vplivajo na odpornost lesa. V ta namen smo preizkusili in optimizirali »Test lesne paste«, pri katerem se za oceno zaviralnega učinka lesnih komponent na rast gliv v hranilno gojišče doda lesni prah. Postopek je bilo treba prilagoditi razpoložljivi laboratorijski opremi in za izvedbo v okviru študijskega procesa na Oddelku za lesarstvo (Biotehniška fakulteta, Univerza v Ljubljani). S testiranjem preraščanja gliv na hranilnih gojiščih z dodanim smrekovim prahom smo ocenjevali uporabo različnih metod mletja in hranilnih gojišč ter možnosti analize podatkov. Ugotovili smo, da je hranilno gojišče s krompirjevim dekstroznim agarjem primerna alternativa gojišču iz sladnega agarja. Izkazalo se je, da velikost frakcije lesnega prahu nima bistvenega vpliva na zaviranje rasti gliv, zato je za test možno uporabiti tudi manj fine frakcije, ki jih dobimo z manj zamudnimi postopki mletja. Analizo slik in modeliranje logističnih krivulj rasti smo opravili z uporabo prosto dostopne programske opreme (ImageJ in R-Commander), ki je učinkovita alternativa plačljivim orodjem. Čeprav pri vizualizaciji hitrosti rasti z uporabo logističnih stopenj rasti izgubimo del informacije o dinamiki rasti, se je ta način izkazal za bolj preglednega v primerjavi s prikazom rastnih krivulj.
Keywords:inhibicija, kemija lesa, hranilno gojišče, glive bele trohnobe, glive rjave trohnobe, logistična stopnja rasti


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