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Title:The advantages of flow cytometry in comparison to fluorometric measurement in algal toxicity test
Authors:ID Eleršek, Tina (Author)
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URL URL - Source URL, visit http://eprints.gozdis.si/id/eprint/14
 
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Language:English
Typology:1.01 - Original Scientific Article
Organization:Logo NIB - National Institute of Biology
Abstract:Fluorometric measurements in algal toxicity tests are very commonly used as surrogate parameters for algal biomass. Although, fluorometry is a powerful technique, we have demonstrated that it is not suitable for determination of toxic effects of chemicals, which alter the fluorescence spectra. We recommendthe flow cytometry as the best technique for detecting algal and/or cyanobacterial cell count and fluorescence per cell. Flow cytometry has many advantages: little volume of algal/cyanobacterial sample required, suitable also for little algal/cyanobacterial cells, distinction between live and dead cells. Furthermore, flow cytometry reveals the early changes in fluorescence spectra as a consequence of the specific chemicals presence or stress, even though the cell count is not yet affected (an early marker for ecotoxicology testing).
Keywords:algae, cyanobacteria, ecotoxicity test, fluorescence, flow cytometry
Publication status:Published
Publication version:Version of Record
Publication date:01.01.2012
Year of publishing:2012
Number of pages:str. 3-11
Numbering:Vol. 55, št. 2
PID:20.500.12556/DiRROS-21555 New window
UDC:579
ISSN on article:1408-3671
COBISS.SI-ID:2712399 New window
Note:Besedilo samo v angl.;
Publication date in DiRROS:26.02.2025
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Downloads:558
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Record is a part of a journal

Title:Acta biologica slovenica : ABS
Shortened title:Acta biol. slov.
Publisher:Društvo biologov Slovenije
ISSN:1408-3671
COBISS.SI-ID:68526592 New window

Document is financed by a project

Funder:EC - European Commission
Project number:265264
Name:Fate and effects of cytostatic pharmaceuticals in the environment and the identification of biomarkers for and improved risk assessment on environmental exposure
Acronym:CYTOTHREAT

Licences

License:CC BY-SA 4.0, Creative Commons Attribution-ShareAlike 4.0 International
Link:http://creativecommons.org/licenses/by-sa/4.0/
Description:This Creative Commons license is very similar to the regular Attribution license, but requires the release of all derivative works under this same license.

Secondary language

Language:Slovenian
Title:Prednosti merjenja s pretočno citometrijo v primerjavi s fluorimetričnim merjenjem v strupenostnih testih z algami
Abstract:V strupenostih testih z algami se zelo pogosto izvajajo fluorometrične meritvekot nadomestni parameter za biomaso alg. Čeprav je fluorimetrija zelo uporabna tehnika, smo pokazali, da ni primerna za določanje strupenih učinkov kemikalij, ki spremenijo spekter fluorescence. Za določanje števila alg in/alicianobakterij in njihove fluorescence na celico priporočamo uporabo pretočne citometrije. Uporaba pretočne citometrije za zaznavanje alg in/ali cianobakterij ima mnogo prednosti: majhni volumni vzorca alg/cianobakterij, primerno tudi za majhne celice alg/cianobakterij, razlikovanje med živimi in mrtvimi celicami. Poleg tega pretočna citometrija razkrije zgodnje spremembe vspektru fluorescence, ki so posledica prisotnosti specifičnih kemikalij oz. stresa, čeprav število celic še ni spremenjeno (zgodnji marker v ekotoksikoloških testiranjih).
Keywords:alge, cianobakterije, ekotoksikološki test, fluorescenca, pretočna citometrija


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