| Title: | Modulation of the proteasome pathway by nano-curcumin and curcumin in retinal pigment epithelial cells |
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| Authors: | ID Carvalho, J. Emanuel Ramos de (Author)
ID Verwoert, Milan T. (Author)
ID Vogels, Ilse M.C. (Author)
ID Schipper-Krom, Sabine (Author)
ID Noorden, Cornelis J. F. van (Author)
ID Reits, Eric A. (Author)
ID Klaassen, Ingeborg (Author)
ID Schlingemann, Reinier O. (Author) |
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| Files: |  PDF - Presentation file, download (737,80 KB)
MD5: 6596BA8506A67F002E66E6C4C9B566EE
 URL - Source URL, visit https://doi.org/10.1159/000481261
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| Language: | English |
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| Typology: | 1.01 - Original Scientific Article |
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| Organization: |  NIB - National Institute of Biology
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| Abstract: | Introduction: Curcumin has multiple biological effects including the modulation of protein homeostasis by the ubiquitin-proteasome system. The purpose of this study was to assess the in vitro cytotoxic and oxidative effects of nano-curcumin and standard curcumin and characterize their effects on proteasome regulation in retinal pigment epithelial (RPE) cells. Methods: Viability, cell cycle progression, and reactive oxygen species (ROS) production were determined after treatment with nano-curcumin or curcumin. Subsequently, the effects of nano-curcumin and curcumin on proteasome activity and the gene and protein expression of proteasome subunits PA28α, α7, β5, and β5i were assessed. Results: Nano-curcumin (5-100 μM) did not show significant cytotoxicity or anti-oxidative effects against H2O2-induced oxidative stress, whereas curcumin (≥10 μM) was cytotoxic and a potent inducer of ROS production. Both nano-curcumin and curcumin induced changes in proteasome-mediated proteolytic activity characterized by increased activity of the proteasome subunits β2 and β5i/β1 and reduced activity of β5/β1i. Likewise, nano-curcumin and curcumin affected mRNA and protein levels of household and immunoproteasome subunits. Conclusions: Nano-curcumin is less toxic to RPE cells and less prone to induce ROS production than curcumin. Both nano-curcumin and curcumin increase proteasome-mediated proteolytic activity. These results suggest that nano-curcumin may be regarded as a proteasome-modulating agent of limited cytotoxicity for RPE cells.
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| Keywords: | curcumin, nano-curcumin, proteasome, retinal pigment epithelium |
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| Publication status: | Published |
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| Publication version: | Version of Record |
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| Publication date: | 01.01.2018 |
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| Year of publishing: | 2018 |
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| Number of pages: | str. 98-109 |
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| Numbering: | Vol. 59, no. 2 |
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| PID: | 20.500.12556/DiRROS-21552  |
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| UDC: | 617.7 |
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| ISSN on article: | 0030-3747 |
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| DOI: | 10.1159/000481261 |
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| COBISS.SI-ID: | 4640591 |
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| Publication date in DiRROS: | 26.02.2025 |
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| Views: | 626 |
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| Downloads: | 399 |
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