| Title: | Effect of castration method on porcine skeletal muscle fiber traits and transcriptome profiles |
|---|
| Authors: | ID Poklukar Žnidaršič, Klavdija (Author)
ID Erbežnik, Anja (Author)
ID Fazarinc, Gregor (Author)
ID Kress, Kevin (Author)
ID Batorek Lukač, Nina (Author)
ID Škrlep, Martin (Author)
ID Stefanski, Volker (Author)
ID Čandek-Potokar, Marjeta (Author)
ID Vrecl, Milka (Author) |
|---|
| Files: |  URL - Source URL, visit https://www.sciencedirect.com/science/article/pii/S2451943X24000504?via%3Dihub
 PDF - Presentation file, download (2,46 MB)
MD5: A3556E7B24D43CF9CEC42D3800122204
|
|---|
| Language: | English |
|---|
| Typology: | 1.01 - Original Scientific Article |
|---|
| Organization: |  KIS - Agricultural Institute of Slovenia
|
|---|
| Abstract: | This study examined the effects of immunocastration and surgical castration on the histomorphometric and transcriptome traits of the porcine skeletal muscle. We hypothesized that the differences in duration of androgen deprivation resulting from different castration methods influence skeletal muscle biology in a muscle-specific manner. This was tested by analyzing samples of m. longissimus dorsi (LD) and m. semispinalis capitis (SSC) from immunocastrated (IC; n = 12), entire male (EM; n = 12), and surgically castrated (SC; n = 12) pigs using enzyme/immunohistochemical classification and histomorphometric analysis of myofibers, quantitative PCR, and RNA sequencing. The results confirmed the distinctive histomorphometric profiles of LD and SSC and the castration method related muscle-specific effects at the histomorphometric and transcriptome levels. Long-term androgen deficiency (surgical castration) significantly reduced the proportion of fast-twitch type IIa myofibers in LD (P < 0.05), whereas short-term androgen deprivation (immunocastration) reduced the cross-sectional area of oxidative type I myofibers in SSC (P < 0.05). At the transcriptional level, glycolytic LD adapted to long- and short-term androgen deprivation by upregulating genes controlling myoblast proliferation and differentiation to maintain fiber size. In contrast, increased protein degradation through the ubiquitin ligase-mediated atrophy pathway (significantly increased TRIM63 and FBXO32 expression; P < 0.05) could underly reduced crosssectional area of type I myofibers in the oxidative SSC in IC. Potential candidate genes (HK2, ARID5B, SERPINE1, and SCD) linked to specific metabolic profiles and meat quality traits were also identified in IC, providing a foundation for studying the effects of immunocastration on skeletal muscle fiber and carcass/meat quality traits. |
|---|
| Keywords: | sceletal muscle, Logissimus dorsi, Semispinalis capitis, castration method, genne expression, pig |
|---|
| Publication status: | Published |
|---|
| Publication version: | Version of Record |
|---|
| Publication date: | 26.07.2024 |
|---|
| Year of publishing: | 2024 |
|---|
| Number of pages: | str. 1-13 |
|---|
| Numbering: | Vol. 25, [article no.] 100383 |
|---|
| PID: | 20.500.12556/DiRROS-21130  |
|---|
| UDC: | 636.4.09:611 |
|---|
| ISSN on article: | 2451-943X |
|---|
| DOI: | 10.1016/j.vas.2024.100383 |
|---|
| COBISS.SI-ID: | 203362563 |
|---|
| Note: | Nasl. z nasl. zaslona;
Opis vira z dne 1. 8. 2024;
|
|---|
| Publication date in DiRROS: | 31.12.2024 |
|---|
| Views: | 52 |
|---|
| Downloads: | 27 |
|---|
| Metadata: |    |
|---|
|
:
|
Copy citation |
|---|
| | | | Share: |  |
|---|
Hover the mouse pointer over a document title to show the abstract or click
on the title to get all document metadata. |
