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Title:Data mining of Saccharomyces cerevisiae mutants engineered for increased tolerance towards inhibitors in lignocellulosic hydrolysates
Authors:ID Cámara, Elena (Author)
ID Olsson, Lisbeth (Author)
ID Zrimec, Jan (Author)
ID Zelezniak, Aleksej (Author)
ID Geijer, Cecilia (Author)
ID Nygård, Yvonne (Author)
Files:URL URL - Source URL, visit https://www.sciencedirect.com/science/article/pii/S073497502200043X?via%3Dihub
 
.pdf PDF - Presentation file, download (3,76 MB)
MD5: 51770C6B28417F78677B1778B5D38DDB
 
URL URL - Source URL, visit https://doi.org/10.1016/j.biotechadv.2022.107947
 
Language:English
Typology:1.02 - Review Article
Organization:Logo NIB - National Institute of Biology
Abstract:The use of renewable plant biomass, lignocellulose, to produce biofuels and biochemicals using microbial cell factories plays a fundamental role in the future bioeconomy. The development of cell factories capable of efficiently fermenting complex biomass streams will improve the cost-effectiveness of microbial conversion processes. At present, inhibitory compounds found in hydrolysates of lignocellulosic biomass substantially influence the performance of a cell factory and the economic feasibility of lignocellulosic biofuels and chemicals. Here, we present and statistically analyze data on Saccharomyces cerevisiae mutants engineered for altered tolerance towards the most common inhibitors found in lignocellulosic hydrolysates: acetic acid, formic acid, furans, and phenolic compounds. We collected data from 7971 experiments including single overexpression or deletion of 3955 unique genes. The mutants included in the analysis had been shown to display increased or decreased tolerance to individual inhibitors or combinations of inhibitors found in lignocellulosic hydrolysates. Moreover, the data included mutants grown on synthetic hydrolysates, in which inhibitors were added at concentrations that mimicked those of lignocellulosic hydrolysates. Genetic engineering aimed at improving inhibitor or hydrolysate tolerance was shown to alter the specific growth rate or length of the lag phase, cell viability, and vitality, block fermentation, and decrease product yield. Different aspects of strain engineering aimed at improving hydrolysate tolerance, such as choice of strain and experimental set-up are discussed and put in relation to their biological relevance. While successful genetic engineering is often strain and condition dependent, we highlight the conserved role of regulators, transporters, and detoxifying enzymes in inhibitor tolerance. The compiled meta-analysis can guide future engineering attempts and aid the development of more efficient cell factories for the conversion of lignocellulosic biomass.
Publication status:Published
Publication version:Version of Record
Publication date:01.08.2022
Year of publishing:2022
Number of pages:str. 1-14
Numbering:Vol. 57
PID:20.500.12556/DiRROS-20101 New window
UDC:60:004
ISSN on article:0734-9750
DOI:10.1016/j.biotechadv.2022.107947 New window
COBISS.SI-ID:120164867 New window
Note:Št. članka: 107947;
Publication date in DiRROS:05.08.2024
Views:484
Downloads:448
Metadata:XML DC-XML DC-RDF
:
CÁMARA, Elena, OLSSON, Lisbeth, ZRIMEC, Jan, ZELEZNIAK, Aleksej, GEIJER, Cecilia and NYGÅRD, Yvonne, 2022, Data mining of Saccharomyces cerevisiae mutants engineered for increased tolerance towards inhibitors in lignocellulosic hydrolysates. Biotechnology advances [online]. 2022. Vol. 57, p. 1–14. [Accessed 21 April 2025]. DOI 10.1016/j.biotechadv.2022.107947. Retrieved from: https://dirros.openscience.si/IzpisGradiva.php?lang=eng&id=20101
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Record is a part of a journal

Title:Biotechnology advances
Shortened title:Biotechnol. adv.
Publisher:Pergamon
ISSN:0734-9750
COBISS.SI-ID:25101568 New window

Document is financed by a project

Funder:Other - Other funder or multiple funders
Project number:P47509–1
Funder:Other - Other funder or multiple funders
Project number:NNF19OC0055044
Name:Novo Nordisk Foundation

Licences

License:CC BY-NC-ND 4.0, Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
Link:http://creativecommons.org/licenses/by-nc-nd/4.0/
Description:The most restrictive Creative Commons license. This only allows people to download and share the work for no commercial gain and for no other purposes.

Secondary language

Language:Slovenian
Keywords:lignocelulozna hidroliza, kvasovke, podatkovno rudarjenje, bioinformatika


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