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Title:The efficient method for simultaneous monitoring of the culturable as well as nonculturable airborne microorganisms
Authors:ID Hubad, Barbara (Author)
ID Lapanje, Aleš (Author)
Files:.pdf PDF - Presentation file, download (436,95 KB)
MD5: 88B4E2499807F7F20BA1115A0E27350D
 
URL URL - Source URL, visit https://doi.org/10.1371/journal.pone.0082186
 
Language:English
Typology:1.01 - Original Scientific Article
Organization:Logo NIB - National Institute of Biology
Abstract:Cultivation-based microbiological methods are a gold standard for monitoring of airborne micro-organisms to determine the occupational exposure levels or transmission paths of a particular infectious agent. Some highly contagious microorganisms are not easily culturable but it is becoming evident that cultivation and molecular methods are complementary and in these cases highly relevant. We report a simple and efficient method for sampling and analyzing airborne bacteria with an impactor-type high-flow-rate portable air sampler, currently used for monitoring culturable bacteria or fungi. A method is reported for extraction of nucleic acids from impacted cells without prior cultivation and using agarose as a sampling matrix. The DNA extraction efficiency was determined in spiked samples and, samples taken from a wastewater treatment plant and an alpine area. The abundance, diversity and quantity of total bacteria were analysed by a quantitative polymerase chain reaction, and by construction and analysis of clone libraries. The method does not interfere with downstream PCR analysis and can cover the gap between traditional culture and molecular techniques of bioaerosol monitoring.
Publication status:Published
Publication version:Version of Record
Publication date:20.12.2013
Year of publishing:2013
Number of pages:Str. 1-9; e82186
Numbering:Vol. 8, št. 12
PID:20.500.12556/DiRROS-20024 New window
UDC:579.6
ISSN on article:1932-6203
DOI:10.1371/journal.pone.0082186 New window
COBISS.SI-ID:3029583 New window
Note:avtorski PDF; e82186;
Publication date in DiRROS:02.08.2024
Views:509
Downloads:228
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HUBAD, Barbara and LAPANJE, Aleš, 2013, The efficient method for simultaneous monitoring of the culturable as well as nonculturable airborne microorganisms. PloS one [online]. 2013. Vol. 8, no. 12, p. 1–9; e82186. [Accessed 21 April 2025]. DOI 10.1371/journal.pone.0082186. Retrieved from: https://dirros.openscience.si/IzpisGradiva.php?lang=eng&id=20024
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Record is a part of a journal

Title:PloS one
Publisher:Public Library of Science
ISSN:1932-6203
COBISS.SI-ID:2005896 New window

Document is financed by a project

Funder:EC - European Commission
Project number:261810
Name:The lab-free CBRN detection device for the identification of biological pathogens on nucleic acid and immunological level as lab-on-a-chip system applying multisensor technologies
Acronym:MULTISENSE CHIP
Funder:EC - European Commission
Project number:312139
Name:Integrated Biotechnological Solutions for Combating Marine Oil Spills
Acronym:KILL●SPILL
Funder:Other - Other funder or multiple funders
Project number:P-MR-07/55
Funder:EC - European Commission
Funding programme:the European Commission Seventh Framework program
Project number:A-1087-RT-GC
Name:Patch

Licences

License:CC BY 4.0, Creative Commons Attribution 4.0 International
Link:http://creativecommons.org/licenses/by/4.0/
Description:This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.

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