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Title:CellTracker Green labelling vs. Rose Bengal staining: CTG wins by points in distinguishing living from dead anoxia-impacted copepods and nematods
Authors:ID Grego, Mateja (Author)
ID Stachowitsch, Michael (Author)
ID De Troch, Marleen (Author)
ID Riedel, Bettina (Author)
Files:.pdf PDF - Presentation file, download (1,74 MB)
MD5: 976BF6DC98EC1365D7F821B8FDCB53FC
 
URL URL - Source URL, visit https://doi.org/10.5194/bg-10-4565-2013
 
Language:English
Typology:1.01 - Original Scientific Article
Organization:Logo NIB - National Institute of Biology
Abstract:Hypoxia and anoxia have become a key threat to shallow coastal seas. Much is known about their impact on macrofauna, less on meiofauna. In an attempt to shed more light on the latter group, in particular from a process-oriented view, we experimentally induced short-term anoxia (1 week) in the northern Adriatic Sea (Mediterranean) and examined the two most abundant meiofauna taxa – harpacticoid copepods and nematodes. Both taxa also represent different ends of the tolerance spectrum, with copepods being the most sensitive and nematodes among the most tolerant. We compared two methods: CellTracker Green (CTG) – new labelling approach for meiofauna – with the traditional rose bengal (RB) staining method. CTG binds to active enzymes and therefore colours live organisms only. The two methods show considerable differences in the number of living and dead individuals of both meiofauna taxa. Generally, RB will stain dead but not yet decomposed copepods and nematodes equally as it does live ones. Specifically, RB significantly overestimated the number of living copepods in all sediment layers in anoxic samples, but not in any normoxic samples. In contrast, for nematodes, the methods did not show such a clear difference between anoxia and normoxia. RB overestimated the number of living nematodes in the top sediment layer of normoxic samples, which implies an overestimation of the overall live nematofauna. For monitoring and biodiversity studies, the RB method might be sufficient, but for more precise quantification of community degradation, especially after an oxygen depletion event, CTG labelling is a better tool. Moreover, it clearly highlights the surviving species within the copepod or nematode community. As already accepted for foraminiferal research, we demonstrate that the CTG labelling is also valid for other meiofauna groups.
Keywords:morje, plankton, kopepodi, živalski plankton, trofični status, morski plankton, CTG, CellTracker Green
Geographic coverage:Jadransko morje;
Publication status:Published
Publication version:Version of Record
Publication date:09.07.2013
Year of publishing:2013
Number of pages:str. 4565-4575
Numbering:Vol. 10, no. 7
PID:20.500.12556/DiRROS-20017 New window
UDC:574
ISSN on article:1726-4170
DOI:10.5194/bg-10-4565-2013 New window
COBISS.SI-ID:2809423 New window
Note:Izv. znanstv. članek; Predčasna objava DOI v BGD februarja 2013, BG jul. 2013; končna objava v BG dne 10. julija 2013;
Publication date in DiRROS:02.08.2024
Views:489
Downloads:303
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GREGO, Mateja, STACHOWITSCH, Michael, DE TROCH, Marleen and RIEDEL, Bettina, 2013, CellTracker Green labelling vs. Rose Bengal staining: CTG wins by points in distinguishing living from dead anoxia-impacted copepods and nematods. Biogeosciences [online]. 2013. Vol. 10, no. 7, p. 4565–4575. [Accessed 21 April 2025]. DOI 10.5194/bg-10-4565-2013. Retrieved from: https://dirros.openscience.si/IzpisGradiva.php?lang=eng&id=20017
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Record is a part of a journal

Title:Biogeosciences
Shortened title:Biogeosciences
Publisher:Copernicus
ISSN:1726-4170
COBISS.SI-ID:3667320 New window

Document is financed by a project

Funder:FWF - Austrian Science Fund
Project number:P21542-B17
Funder:Other - Other funder or multiple funders
Project number:SI 22/2009
Name:Bilateral Slovenian Austrian Scientific Technical Cooperation project
Acronym:OEAD
Funder:Other - Other funder or multiple funders
Funding programme:the Special Research Fund of Ghent University
Project number:01GA1911W
Acronym:GOA

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License:CC BY 3.0, Creative Commons Attribution 3.0 Unported
Link:https://creativecommons.org/licenses/by/3.0/deed.en
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