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Title:Loop-mediated isothermal amplification of specific endoglucanase gene sequence for detection of the bacterial wilt pathogen Ralstonia solanacearum
Authors:ID Lenarčič, Rok (Author)
ID Morisset, Dany (Author)
ID Pirc, Manca (Author)
ID Llop, Pablo (Author)
ID Ravnikar, Maja (Author)
ID Dreo, Tanja (Author)
Files:.pdf PDF - Presentation file, download (339,81 KB)
MD5: 3C332C31F860C87CA71C09091416A330
 
URL URL - Source URL, visit https://doi.org/10.1371/journal.pone.0096027
 
Language:English
Typology:1.01 - Original Scientific Article
Organization:Logo NIB - National Institute of Biology
Abstract:The increased globalization of crops production and processing industries also promotes the side-effects of more rapid and efficient spread of plant pathogens. To prevent the associated economic losses, and particularly those related to bacterial diseases where their management relies on removal of the infected material from production, simple, easy-to-perform, rapid and cost-effective tests are needed. Loop-mediated isothermal amplification (LAMP) assays that target 16S rRNA, fliC and egl genes were compared and evaluated as on-site applications. The assay with the best performance was that targeted to the egl gene, which shows high analytical specificity for diverse strains of the betaproteobacterium Ralstonia solanacearum, including its non-European and non-race 3 biovar 2 strains. The additional melting curve analysis provides confirmation of the test results. According to our extensive assessment, the egl LAMP assay requires minimum sample preparation (a few minutes of boiling) for the identification of pure cultures and ooze from symptomatic material, and it can also be used in a high-throughput format in the laboratory. This provides sensitive and reliable detection of R. solanacearum strains of different phylotypes.
Keywords:plant pathogens, bacterial diseases
Publication status:Published
Publication version:Version of Record
Publication date:24.04.2014
Year of publishing:2014
Number of pages:str. 1-8
Numbering:Vol. 9, iss. 4
PID:20.500.12556/DiRROS-20003 New window
UDC:581.2
ISSN on article:1932-6203
DOI:10.1371/journal.pone.0096027 New window
COBISS.SI-ID:3114575 New window
Note:Nasl. z nasl. zaslona; Opis vira z dne 29. 4. 2014;
Publication date in DiRROS:02.08.2024
Views:496
Downloads:216
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LENARČIČ, Rok, MORISSET, Dany, PIRC, Manca, LLOP, Pablo, RAVNIKAR, Maja and DREO, Tanja, 2014, Loop-mediated isothermal amplification of specific endoglucanase gene sequence for detection of the bacterial wilt pathogen Ralstonia solanacearum. PloS one [online]. 2014. Vol. 9, no. 4, p. 1–8. [Accessed 28 March 2025]. DOI 10.1371/journal.pone.0096027. Retrieved from: https://dirros.openscience.si/IzpisGradiva.php?lang=eng&id=20003
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Record is a part of a journal

Title:PloS one
Publisher:Public Library of Science
ISSN:1932-6203
COBISS.SI-ID:2005896 New window

Document is financed by a project

Funder:EC - European Commission
Project number:245047
Name:Developing quarantine pest detection methods for use by national plant protection organizations (NPPO) and inspection services
Acronym:QDETECT
Funder:ARIS - Slovenian Research and Innovation Agency
Project number:L1-3642
Name:Developing simple, rapid and on-site methods for plant pathogens detection

Licences

License:CC BY 4.0, Creative Commons Attribution 4.0 International
Link:http://creativecommons.org/licenses/by/4.0/
Description:This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.

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