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Title:Salicylic acid is an indispensable component of the Ny-1 resistance-gene-mediated response against Potato virus Y infection in potato
Authors:ID Baebler, Špela (Author)
ID Witek, Kamil (Author)
ID Petek, Marko (Author)
ID Stare, Katja (Author)
ID Tušek-Žnidarič, Magda (Author)
ID Pompe Novak, Maruša (Author)
ID Renaut, Jenny (Author)
ID Szajko, K. (Author)
ID Strzelczyk-Żyta, D. (Author)
ID Marczewski, W. (Author)
ID Morgiewicz, Karolina (Author)
ID Gruden, Kristina (Author)
ID Hennig, Jacek (Author)
Files:.pdf PDF - Presentation file, download (4,98 MB)
MD5: 0EC015FDFB4E88E185EAFD73F8C7FA8F
 
URL URL - Source URL, visit http://dx.doi.org/10.1093/jxb/ert447
 
Language:English
Typology:1.01 - Original Scientific Article
Organization:Logo NIB - National Institute of Biology
Abstract:The purpose of the study was to investigate the role of salicylic acid (SA) signalling in Ny-1-mediated hypersensitive resistance (HR) of potato (Solanum tuberosum L.) to Potato virus Y (PVY). The responses of the Ny-1 allele in the Rywal potato cultivar and transgenic NahG-Rywal potato plants that do not accumulate SA were characterized at the cytological, biochemical, transcriptome, and proteome levels. Analysis of noninoculated and inoculated leaves revealed that HR lesions started to develop from 3 d post inoculation and completely restricted the virus spread. At the cytological level, features of programmed cell death in combination with reactive oxygen species burst were observed. In response to PVY infection, SA was synthesized de novo. The lack of SA accumulation in the NahG plants led to the disease phenotype due to unrestricted viral spreading. Grafting experiments show that SA has a critical role in the inhibition of PVY spreading in parenchymal tissue, but not in vascular veins. The whole transcriptome analysis confirmed the central role of SA in orchestrating Ny-1-mediated responses and showed that the absence of SA leads to significant changes at the transcriptome level, including a delay in activation of expression of genes known to participate in defence responses. Moreover, perturbations in the expression of hormonal signalling genes were detected, shown as a switch from SA to jasmonic acid/ethylene signalling. Viral multiplication in the NahG plants was accompanied by downregulation of photosynthesis genes and activation of multiple energy-producing pathways.
Keywords:plant-pathogen interactions, Potato virus Y, salicylic acid, whole transcriptome analysis
Publication status:Published
Publication version:Version of Record
Publication date:01.03.2014
Year of publishing:2014
Number of pages:str. 1095-1109
Numbering:Vol. 65, iss. 4
PID:20.500.12556/DiRROS-19997 New window
UDC:578
ISSN on article:0022-0957
DOI:10.1093/jxb/ert447 New window
COBISS.SI-ID:3027535 New window
Publication date in DiRROS:01.08.2024
Views:463
Downloads:299
Metadata:XML DC-XML DC-RDF
:
BAEBLER, Špela, WITEK, Kamil, PETEK, Marko, STARE, Katja, TUŠEK-ŽNIDARIČ, Magda, POMPE NOVAK, Maruša, RENAUT, Jenny, SZAJKO, K., STRZELCZYK-ŻYTA, D., MARCZEWSKI, W., MORGIEWICZ, Karolina, GRUDEN, Kristina and HENNIG, Jacek, 2014, Salicylic acid is an indispensable component of the Ny-1 resistance-gene-mediated response against Potato virus Y infection in potato. Journal of Experimental Botany [online]. 2014. Vol. 65, no. 4, p. 1095–1109. [Accessed 28 March 2025]. DOI 10.1093/jxb/ert447. Retrieved from: https://dirros.openscience.si/IzpisGradiva.php?lang=eng&id=19997
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Record is a part of a journal

Title:Journal of Experimental Botany
Shortened title:J. Exp. Bot.
Publisher:Clarendon Press
ISSN:0022-0957
COBISS.SI-ID:5141514 New window

Document is financed by a project

Funder:ARIS - Slovenian Research and Innovation Agency
Project number:P4-0165-2009
Name:Biotehnologija in sistemska biologija rastlin
Funder:ARIS - Slovenian Research and Innovation Agency
Project number:J4-4165-2011
Name:Kompromisi obrambe in razvoja v večtrofični interakciji med krompirjem in dvema glavnima škodljivcema
Funder:Other - Other funder or multiple funders
Project number:N N302 114737
Funder:Other - Other funder or multiple funders
Funding programme:SlovenianPolish bilateral project
Project number:2008-5463-BILATERAL
Acronym:FEBS and CEEPUS
Funder:Other - Other funder or multiple funders
Project number:FA0806
Name:COST actions
Funder:Other - Other funder or multiple funders
Project number:FA0603
Name:COST actions

Licences

License:CC BY 3.0, Creative Commons Attribution 3.0 Unported
Link:https://creativecommons.org/licenses/by/3.0/deed.en
Description:You are free to reproduce and redistribute the material in any medium or format. You are free to remix, transform, and build upon the material for any purpose, even commercially. You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use. You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.

Secondary language

Language:Latin
Keywords:Potyviridae, Solanum tuberosum


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